升清胶囊对胆色素结石豚鼠肝细胞核因子-κB蛋白表达的影响  

Effects of Shengqing Capsules on expression of nuclear factor-κB protein in liver cells of guinea pigs with pigment gallstone

作　　者：顾宏刚[1] 张静喆[1] 章学林[1] 刘建文[1] 李婷[1] 朱培庭[1] 

机构地区：[1]上海中医药大学龙华医院胆道外科,上海200032

出　　处：《中西医结合学报》2006年第5期518-521,共4页Journal of Chinese Integrative Medicine

基　　金：上海市卫生系统百人计划资助项目(No.03HBR-29);上海市重点学科建设资助项目(No.T0304)

摘　　要：目的:探讨疏肝利胆中药升清胶囊防治胆色素结石的作用机制。方法:用原代细胞培养方法建立胆色素结石豚鼠肝细胞模型;采用血清药理学方法制备不同浓度含升清胶囊药物血清;建立脂多糖刺激下体外培养原代肝细胞高核转录因子-κB(nuclearfactor-κB,NF-κB)蛋白表达的实验模型,用Western-blotting检测不同浓度含药血清对于NF-κB蛋白表达的影响。结果:升清胶囊能降低脂多糖刺激引起的NF-κB蛋白的表达;随着用药浓度的增加,升清胶囊对脂多糖刺激引起的NF-κB蛋白表达的抑制作用也越强。结论:疏肝利胆中药升清胶囊能降低脂多糖刺激引起的NF-κB蛋白的表达,可能是其防治胆色素结石成石及胆道炎症反应的作用机制之一。Objective： To study the mechanisms of Shengqing Capsules, a compound Chinese herbal medicine for dispersing stagnated liver qi and promoting bile flow, in prevention and treatment of pigment gallstone. Methods： Liver cells from guinea pigs with pigment gallstone were primarily cultured in vitro. The serums containing different concentrations of Shengqing Capsules were prepared by serum pharmacological method. The primary cultured hepatocytes with excessive expression of nuclear factor-κB （NF-κB） were stimulated with lipopolysaccharides （LPS） and the effects of the drug-containing serums on the NF-κB expression were detected by Western-blotting. Results： Shengqing Capsules down-regulated the NF-κB expression increased by LPS stimulation. The effect is dose-dependent. Conclusion： Shengqing Capsules can down-regulate the NF-κB expression increased by LPS stimulation. It may be one of the important mechanisms of this Chinese herbal medicine in prevention and treatment for pigment gallstone formation and the inflammatory reaction in biliary tract.

关 键 词：肝细胞 核转录因子-ΚB 豚鼠 胆结石 

分 类 号：R285.5[医药卫生—中药学]