可注射组织工程材料壳聚糖-β-磷酸三钙/富血小板血浆对体外培养骨髓基质干细胞增殖的影响  

Effect of Injectable Scaffolds Material Chitosan- beta-tricalcium Phosphate with Platelet-rich plasma on Proliferation of Bone Marrow Stromal Cells in Vitro

作　　者：程文俊[1] 金丹[1] 裴国献[1] 曾宪利[1] 刘华[2] 周长忍[2] 相大勇[1] 

机构地区：[1]南方医科大学南方医院创伤骨科中心,广东省广州市510515 [2]暨南大学材料科学与工程系

出　　处：《中国骨与关节损伤杂志》2006年第9期714-716,共3页Chinese Journal of Bone and Joint Injury

基　　金：国家高科技研究发展计划(863)重点项目(2003AA205010);国家自然科学基金(30300367);广州市科技攻关重大专项子课题(200481-E0031)

摘　　要：目的研究富血小板血浆(PRP)与可注射性材料壳聚糖-β-磷酸三钙(β-TCP)复合后对体外培养骨髓基质干细胞(BM-SCs)的增殖作用,探讨PRP作为壳聚糖-β-TCP生长因子来源的可行性。方法将体外培养并扩增的中国青山羊BMSCs与可注射的壳聚糖-β-TCP支架体外复合培养,分为空白对照组、单纯材料组和PRP/材料组,通过倒置显微镜观察细胞生长情况,MTT法检测材料中PRP对细胞增殖的影响。结果PRP/材料组中体外培养的BMSCs增殖优于单纯材料组和空白对照组(P<0·05),单纯材料组和空白对照组细胞增殖无明显差异。结论复合材料中的PRP能促进体外培养的BMSCs增殖,PRP作为可注射性材料壳聚糖-β-TCP中的生长因子是可行的。Objective To study the effects of platelet- rich plasma （PRP） mixed with injectable scaffolds material chitosan - beta- tricalcium phosphate （beta- TCP） on proliferation of bone marrow stromal cells （BMSCs） in vitro and confirm whether PRP can be used as the growth factor resource of injectable chitosan - beta - TCP. Methods The third passage of BMSCs was cultured in vitro and random - izedly divided into the PRP/chitosan - beta - TCP group, chitosan - beta - TCP group and blank control group respectively. The mor - phology and proliferation of BMSCs were observed by inverted phase contrast microscope. MTT assay was used to evaluate the effects of PRP in Chitosan- beta - TCP on proliferation of BMSCs. Results BMSCs cocultured with PRP/Chitosan - beta - TCP exhibited good growth as observed under inverted phase contrast microscope,significantly different from other two groups. MTT assay showed signifi - cant difference in the number of cells of PRP with other two groups. By both inverted phase- contrast microscope and MTT assay,pro- liferation of BMSCs of chitosan- beta - TCP group and control group had no significant difference. Conclusion PRP in injectable scaffolds material chitosan - beta - TCP can promote BMSCs proliferation, which can be used as growth factor resource of chitosan - beta - TCP.

关 键 词：组织工程 富血小板血浆 生物材料 可注射 

分 类 号：R318.08[医药卫生—生物医学工程]