索他洛尔对离体猪右心室动作电位恢复特性的影响  

Effect of d,1-sotalol on action potential duration restitution properties in isolated swine right ventricle

作　　者：王业松[1] 薜娇洁[1] 胡苑[1] 冯冲[1] 唐安丽[1] 董吁钢[1] 马虹[1] 

机构地区：[1]中山大学附属第一医院心血管内科,广州510080

出　　处：《中华心律失常学杂志》2006年第4期271-275,共5页Chinese Journal of Cardiac Arrhythmias

摘　　要：目的评价索他洛尔对动作电位时限(APD)恢复特性的影响,以及索他洛尔抗心室颤动(室颤)作用是否与APD恢复特性有关。方法7只离体灌注猪的右心室,用微电极技术在基础状态、索他洛尔(1～10 mg/L)灌注和洗脱(1 h)时记录跨膜动作电位。在S\-1S\-2程序刺激和室颤期间,由动作电位复极90％时限(APD\-\{90\})和舒张期构建APD恢复曲线。APD恢复最大斜率(S\-\{max\})由APD恢复曲线所获得的资料计算。结果索他洛尔(5～10mg/L)延长APD\-\{90\},减小S\-1S\-2起搏和室颤的S\-\{max\},致使室颤终止(n=2)、转化为室性心动过速(室速,n=3),室颤不能诱发(基础状态24．4％vs索他洛尔0％)以及室颤周期长度增加。结论索他洛尔延长APD\-\{90\}、减小APD恢复最大斜率而发挥抗室颤作用。Objective To evaluate the effect of d, 1 -sotalol on action potential duration（APD） restitution properties and to establish whether its antifibrillatory effect is determined by the anti-restitution properties. Methods Transmcmbrane action potentials were recorded with the microelectrode technique in seven isolated perfused swine right ventricles during the baseline, d, 1-sotalol（ 1-10 mg/L） and washout period （ 1 hour）. APD restitution curves were construeted by plotting APD at 90% repolarization （ APD90 ） versus diastolic interval （DI） during S1S2 programmed ventricular stimulation and ventricular fibrillation （VF）. The maximal slopes （ Smax ） of APD restitution were calculated with the data obtained from APD restitution curves. Results Sotalol （5 - 10 rag/L） prolonged APD90 and reduced Smax during S1S2 pacing and VF, Accordingly, VF was terminated （ n ： 2 ） , or converted to ventricular tachycardia （ VT, n = 3 ） , or continued at a slower rate （n = 2）. VF inducibility was reduced from 24.4% （baseline） to 0%. Conclusion The sotalol may produce the anti-fibrillatory effect by APD prolongation in parallel with a flattening of the Smax of APD restitution.

关 键 词：动作电位时限 恢复特性 心室颤动 索他洛尔 

分 类 号：R965[医药卫生—药理学]