创伤失血后大鼠单核/巨噬细胞分泌功能变化的异质性及其意义  

Heterogeneity changes in secretion ability of monocyte/macrophage and its significance after trauma hemorrhage in rat

作　　者：尹华华[1] 夏浩[1] 朱京慈[2] 顾长国[1] 徐发良 李磊[1] 

机构地区：[1]第三军医大学大坪医院野战外科研究所一室,重庆400042 [2]第三军医大学护理系,重庆400038 [3]新疆马兰解放军第五四六医院,841700

出　　处：《中国危重病急救医学》2006年第9期523-526,共4页Chinese Critical Care Medicine

基　　金：国家重点基础研究发展规划项目(G1999054203);国家教育部骨干教师资助项目;全军"十五"攻关课题(01MA167)

摘　　要：目的探讨单核/巨噬细胞分泌功能异质性与创伤后机体免疫功能紊乱的相互关系。方法健康Wistar大鼠24只,按随机数字表法分为创伤前及创伤失血后1、4和7d组。分别以放射免疫法测定不同部位单核/巨噬细胞分泌白细胞介素6(IL6)及IL10的含量。结果1生理状态下,腹腔巨噬细胞(PM)IL10分泌量最高,单核细胞(Mo)次之;而Mo的IL6分泌量最高,PM次之;肺泡巨噬细胞(AM)两种细胞因子的分泌量均处于较低水平。2创伤失血后大鼠单核/巨噬细胞各部位分泌功能变化具有差异性。AM的IL6及IL10分泌均显著增加;PM的IL6分泌伤后1d和4d下降,7d上升,略高于伤前;与之相反,伤后1d和4d IL10分泌显著增高,7d降低,略低于伤前。Mo的IL6分泌持续下降,IL10分泌持续上升,至伤后7d达顶峰。结论生理状态下单核/巨噬细胞分泌能力存在异质性,创伤失血后这种异质性表现更为明显,并可能成为创伤后机体分泌功能紊乱的重要原因。Objective To investigate the relationship between the heterogeneity in secretion ability of monocyte （Mo）/macrophage and the immune dysfunction after severe trauma. Methods Twenty-four healthy Wistar rats were randomly divided into normal control group and trauma hemorrhage 1, 4 and 7 days groups. The contents of interleukin - 6 （IL - 6） and IL - 10 secretion of Mo/macrophage from different anatomical regions were determined by radioimmunoassay. Results ①In normal rats, the ability to secrete IL - 6 and IL - 10 was different among alveolar macrophages （AM）, peritoneal macrophages （PM） and Mo. PM showed the highest ability to secrete IL - 10 while Mo had the highest ability to secrete IL - 6.②After trauma hemorrhage, the secretion of IL - 6 and IL - 10 by AM were increased dramatically. On the contrary, the secretion of IL - 6 by PM was declined from the 1 st day to the 4 th day, then increased even over that of the normal control group on the 7 th day, However, the secretion of IL - 10 by PM was significantly elevated on the 1 st day after trauma hemorrhage, peaking on the 4 th day, and only slight lowering was found on the 7 th day. The secretion of IL - 6 by Mo was declined gradually all the time, reaching the lowest point on the 7 th day. On the contrary, the secretion of IL - 10 by Mo was increasing, reaching its peak on the 7 th day. Conclusion The heterogeneity of secretion ability of Mo/macrophage obtained from different anatomical regions is present under normal condition, and is more obvious following a severe injury. This change may play an important role in the immune dysfunction and the development of complications after trauma.

关 键 词：单核细胞 巨噬细胞 异质性 分泌能力 白细胞介素类 

分 类 号：R641[医药卫生—外科学]