乌司他丁对失血性休克大鼠回肠黏膜细胞凋亡的影响  

Effect of ulinastatin on apoptosis in ileal mucosa of rats with hemorrhagic shock

作　　者：蒋龙元[1] 杨炼红[2] 钟娃[1] 何志捷[1] 常建星[1] 黄子通[1] 

机构地区：[1]中山大学附属第二医院急诊科,广州510120 [2]中山大学附属第二医院神经科,广州510120

出　　处：《中国危重病急救医学》2006年第9期542-545,共4页Chinese Critical Care Medicine

基　　金：广东省科技计划项目(2004B30601001)

摘　　要：目的探讨乌司他丁对失血性休克大鼠回肠黏膜细胞凋亡的影响。方法采用前瞻对照动物研究,用改良Chaudry方法制备大鼠失血性休克模型,60min后回输血液和生理盐水进行复苏,部分加用乌司他丁治疗。检测不同时间点血清肿瘤坏死因子α(TNFα)、丙二醛(MDA)含量和回肠黏膜细胞Bax、Bcl2、caspase3蛋白的表达水平。结果大鼠失血性休克及复苏后血清TNFα、MDA含量以及回肠黏膜细胞Bax、caspase3蛋白的表达较正常对照组均明显升高,Bcl2明显降低。复苏后血清TNFα含量下降,MDA含量升高;回肠黏膜细胞Bax、caspase3蛋白的表达均明显下降,Bcl2蛋白明显升高;乌司他丁复苏组上述指标的恢复程度较生理盐水复苏组为好。结论乌司他丁可抑制失血性休克大鼠回肠黏膜细胞的凋亡,对失血性休克大鼠起保护作用。Objective To investigate the effect of ulinastatin on apoptosis in ileal mucosa of rats with hemorrhagic shock. Methods A prospective, controlled animal study was performed. The rat model of hemorrhagic shock was replicated according to method described by Chaudry. After 60 minutes period of bleeding, rats were resuscitated by transfusion of shed blood and normal saline. A part of the animals were additionally treated with ulinastatin. The expression of tumor necrosis factor -α （TNF -α）, malondialdehyde （MDA） content in serum, and expression of Bax, Bcl -2, caspase 3 protein in ileal mucosa were determined at different time points after reperfusion. Results Compared with the normal saline group, the expression levels of TNF -α, MDA content in serum, Bax and caspase 3 protein in ileal mucosa during hemorrhagic shock after resuscitation were significantly increased, while Bcl -2 protein was markedly decreased. After fluid resuscitation, obvious increase in MDA, Bcl -2 protein, significant decrease in the level of TNF -α, the expression of Bax and caspase 3 protein in ileal mucosa were observed in the ulinastatin group compared with normal saline group. Conclusion Ulinastatin has protective effect on rats with hemorrhagic shock by suppressing the apoptosis in ileal mucosa.

关 键 词：乌司他丁 休克 失血性 回肠黏膜 凋亡 大鼠 

分 类 号：R965[医药卫生—药理学]