东北红豆杉及其伤愈组织粗提物中紫杉醇的HPLC-ESI-MS/MS分析研究  

Separation and identification of Taxol in the crude extracts of Taxus cuspidata and its callus culture with HPLC-ESI-MS/MS

作　　者：张洁[1] 段继诚[1] 梁振[1] 张维冰[1] 张丽华[1] 霍玉书[2] 张玉奎[1] 

机构地区：[1]中国科学院大连化学物理研究所国家色谱研究分析中心,辽宁大连116023 [2]德克萨斯大学健康科学中心

出　　处：《药学学报》2006年第9期863-866,共4页Acta Pharmaceutica Sinica

基　　金：中国科学院知识创新工程重要方向性项目(KGCX2-SW-213);国家自然科学基金资助项目(20375040)

摘　　要：目的研究紫杉醇的二级质谱裂解规律,对东北红豆杉及其伤愈组织粗提物中的紫杉醇进行HPLC-ESI-MS/MS分离定性,为天然产物中该化合物的分离分析提供一种快速可靠的方法。方法对HPLC-ESI-MS/MS条件进行优化,研究紫杉醇电喷雾质谱的一级电离规律和二级质谱裂解规律,并结合液相色谱的保留时间判断东北红豆杉及其伤愈组织粗提物中紫杉醇色谱峰的归属。结果详细阐明了紫杉醇的二级质谱裂解规律,并利用HPLC-ESI-MS/MS法准确快速地分离定性了两种粗提物中紫杉醇。结论本方法灵敏度高,快速,选择性高,专属性好,能够作为天然产物及其产品中紫杉醇分离定性的一种快速可靠的技术手段。Aim To study the MS/MS fragmentation mechanism of Taxol, and based on it to establish HPLC-ESI-MS/MS technique to separate and identify Taxol in the crude extracts of Taxus cuspidata and its callus culture, consequently to provide a fast and credible method for the analysis of Taxol in natural products. Methods Optimized the HPLC-ESI-MS/MS parameters for the sample analysis, and then discussed the ionization and cleavage mechanism of Taxol in ESI-MS and ESI-MS/MS, finally identified the Taxol in the samples with retention time, molecular weight and MS/MS spectra. Results Elucidated the MS/MS fragmentation mechanism of Taxol, and developed HPLC-ESI-MS/MS method to analyze Taxol in the two samples. Conclusion The HPLC-ESI-MS/MS method is rapid, highly sensitive and specific, so it is suitable for the separation and identification of Taxol in natural products.

关 键 词：紫杉醇 东北红豆杉 伤愈组织 高效液相色谱一电喷雾质谱联用 

分 类 号：R917[医药卫生—药物分析学]