人参皂苷Rg1和Rb1对UVB照射引起的HaCaT细胞损伤的保护作用  

The protective effects of ginsenoside Rg1 and Rb1 against damage of HaCaT cells by ultraviolet B

作　　者：程俊霖[1] 周黎明[1] 管晓琳[1] 匡湘红[1] 朱玲[1] 

机构地区：[1]四川大学华西医学中心基础药理教研室

出　　处：《药学学报》2006年第9期905-908,共4页Acta Pharmaceutica Sinica

基　　金：四川省科委基金资助项目(02SY029-137)

摘　　要：Aim To investigate the survival rate and the level of HaCaT cells damage with ultraviolet B（UVB） radiation at various doses,and observe the protective effects of ginsenoside Rg1 and Rb1 in vitro.Methods MTT assay was employed to analyze the cell survival rate after UVB radiation of 30,60,90 and 120 mJ·cm-2.The damage of nucleolus and the protective effects of ginsenoside Rg1 and Rb1 were scanned by Hoechst 33258 staining and single cell gel electrophoresis assay（SCGE）.Results It was found that the cell survival rate decreased gradually and the damage of nucleolus aggravated as the radiation dose increased from 30 mJ·cm-2 to 120 mJ·cm-2.At the dose of 20 μg·mL-1,obvious protective effect of ginsenoside Rg1 and Rb1 can be observed against UVB radiation-induced HaCaT cells growth inhibition and nucleolus damage.Conclusion UVB radiation inhibits HaCaT human keratinocytes growth and ginsenoside Rg1 and Rb1 can relief the damage.Aim To investigate the survival rate and the level of HaCaT cells damage with ultraviolet B （UVB） radiation at various doses, and observe the protective effects of ginsenoside Rgl and Rbl in vitro. Methods MTT assay was employed to analyze the cell survival rate after UVB radiation of 30, 60, 90 and 120 mJ·cm^-2 The damage of nucleolus and the protective effects of ginsenoside Rgl and Rbl were scanned by Hoechst 33258 staining and single cell gel electrophoresis assay （SCGE）. Results It was found that the cell survival rate decreased gradually and the damage of nucleolus aggravated as the radiation dose increased from 30 mJ ·cm^-2 to 120 mJ · cm^-2 At the dose of 20 μg ·mL^-1, obvious protective effect of ginsenoside Rgl and Rbl can be observed against UVB radiation-induced HaCaT cells growth inhibition and nucleolus damage. Conclusion UVB radiation inhibits HaCaT human keratinocytes growth and ginsenoside Rgl and Rbl can relief the damage.

关 键 词：HACAT细胞 中波紫外线 人参皂苷 

分 类 号：R282.71[医药卫生—中药学] R965[医药卫生—中医学]