微透析法测定青藤碱大鼠体外血浆蛋白结合率  

Determination in vitro of rat plasma protein binding rate of sinomenine by using microdialysis method

作　　者：张英丰[1] 周莉玲[1] 李锐[1] 

机构地区：[1]广州中医药大学,广东广州510405

出　　处：《药学学报》2006年第9期909-912,共4页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金资助项目(30371783);国家中医药管理局中医药科学技术研究专项资助项目(02-03ZP0047);广东省自然科学基金资助项目(31473)

摘　　要：Aim To determine in vitro the rat plasma protein binding rate by using microdialysis method.Methods The binding rate was determined by using microdialysis probe as sampling tools and zero-net flux method as calibrating method.The regression equation was made by the difference of concentrations between the dialysis sample and the perfusate.The（x-intercept） of regression equation was the free drug concentration（Cf）.The plasma protein binding rate was calculated by using the following equation: f=（C0-Cf）/C0.Result The binding rate was kept relatively stable in the studied concentration range.Conclusion It is feasible that the plasma protein binding rate can be determined by using microdialysis method.Aim To determine in vitro the rat plasma protein binding rate by using microdialysis method. Methods The binding rate was determined by using microdialysis probe as sampling tools and zero-net flux method as calibrating method. The regression equation was made by the difference of concentrations between the dialysis sample and the perfusate. The x-intercept of regression equation was the free drug concentration （Cf）. The plasma protein binding rate was calculated by using the following equation： f = （Co - Cf）/C0. Result The binding rate was kept relatively stable in the studied concentration range. Conclusion It is feasible that the plasma protein binding rate can be determined by using microdialysis method.

关 键 词：青藤碱 血浆蛋白结合率 微透析 零净通量法 

分 类 号：R917[医药卫生—药物分析学]