铜绿假单胞菌生物被膜形成能力与基因型的相关性分析  

Quantification for Biofilm Formation of Pseudomonas aeruginosa Isolates and Their Genotypic Analysis

作　　者：程曦[1] 杨维青[1] 石磊[2] 尹晓琳[3] 谢轶[1] 曾蔚[1] 李心晖[2] 苏建裕[2] 寇亚丽[2] 贾文祥[1] 

机构地区：[1]四川大学华西基础医学与法医学院微生物学教研室,成都610041 [2]华南理工大学食品与生物工程学院 [3]河北医科大学基础医学院

出　　处：《四川大学学报（医学版）》2006年第5期666-669,共4页Journal of Sichuan University(Medical Sciences)

基　　金：国家自然科学基金(批准号30370079);纽约中华医学会基金(CMBNo.82-421)资助

摘　　要：目的探讨铜绿假单胞菌(PA)临床分离株的粘附性、生物被膜形成能力及其与基因型的相关性。方法采用改良的微量平板法对临床分离的48株PA生物被膜的形成进行定量分析;通过肠杆菌基因间重复一致序列-PCR(ERIC-PCR)技术绘制48株PA的指纹图谱,应用计算机软件建立遗传相似性系数矩阵及聚类分析树状图。结果48株临床分离PA菌株生物被膜的形成能力不同。生物被膜形成能力不同的PA在基因型上表现出多态性,PA在17%遗传相似性系数上分为A、B、C、D、E五个基因型,生物被膜形成能力强的PA大都属于D型,其遗传相似性系数为42%。结论绝大多数临床分离的PA株具有较强的生物被膜形成能力;生物被膜形成能力不同的PA在基因型上表现出多态性;生物被膜形成能力较强的PA在基因型上表现出一定的相似性。Objective To probe the correlation between the ability of biofilm formation of Pseudomonas aeruginosa isolates and their genotypes, Methods Forty-eight Pseudomonas aeruginosa isolates were tested for their biofilm formation with a modified microtiter test and were analyzed by enterobacterial repetitive intergenlc consensus PCR （ERIC-PCR）. The percent similarity between their genetic fingerprints and cluster analysis was performed and worked out using computer software. Results Forty-elght Pseudomonas aeruginosa isolates demonstrated different abilities of biofilm formation in vitro. And the 48 isolates with different abilities of biofilm formation showed different genotypes in fingerprints by ERIC-PCR. Among the 48 isolates of Pseudomonas aeruginosa tested, five clusters （A, B,C,D,E）, were identified at the 17% genetic similarity level. The isolates with strong ability of biofilm formation were in D cluster at the 42% genetic similarity level. Conclusion Most of the 48 PA isolates tested formed strong biofilm, and their genotypes were of correlation.

关 键 词：铜绿假单胞菌 生物被膜 基因指纹图谱 基因型 

分 类 号：R378[医药卫生—病原生物学]