巴曲酶对原代培养的大鼠皮层神经元钙激活钾通道的作用研究  

Effect of Batroxobin on K^+ Channel Activated by Ca^(2+) in Primary Culture Rat Cortex Neurons of Rat

作　　者：赖晓晖[1] 朱文梅[1] 徐刚[2] 袁光固[1] 

机构地区：[1]四川大学华西医院神经内科,成都610041 [2]四川省人民医院

出　　处：《四川大学学报（医学版）》2006年第5期687-691,共5页Journal of Sichuan University(Medical Sciences)

摘　　要：目的观察巴曲酶对原代培养新生SD大鼠皮层神经元上钙激活钾通道(Kca)作用。方法采用细胞贴附和内面向外两种膜片钳单通道记录技术检测巴曲酶对Kca作用。结果在钙浴液内,细胞贴附式膜片上,钳制膜电位+30mV,加入不同浓度巴曲酶0.15mmol/L、0.25mmol/L、0.50mmol/L、0.75mmol/L、1.0mmol/L,通道开放概率由0.005分别增加为0.013±0.002、0.082±0.011、0.131±0.012、0.211±0.010、0.062±0.009(P<0.01),在0.75mmol/L以内表现出浓度依赖性。在无钙浴液内,细胞贴附式膜片上,钳制膜电位+50mV,随巴曲酶浓度增加为0.15mmol/L、0.40mmol/L、0.60mmol/L、1.0mmol/L时,通道开放概率由0.005分别增加为0.013±0.001、0.112±0.007、0.193±0.010、0.301±0.009(P<0.05)。6例内面向外式膜片上,钳制膜电位+40mV,分别加入巴曲酶0mmol/L、0.25mmol/L、0.50mmol/L3min后,通道开放概率分别为0.012±0.007、0.011±0.009、0.013±0.008(P>0.05),巴曲酶在胞内Kca开放概率,平均开放/关闭时间,电流幅值均无明显变化。结论巴曲酶通过影响胞内游离钙水平间接调节Kca,可能对神经元有直接保护作用。Objective To investigate the effect of batroxobin on K^＋ channel activated by Ca^2＋ in primary cultured cortex neurons of fetal SD rat. Methods The patch clamp technique of single channel recordings including cell-attach and inslde-out mode was used. Results Extracellular batroxobin activated the Kca. In Ca^2＋ bath solution of cell-attach mode, Vp ＋30 mV, when the concentrations of batroxobin were 0. 15, 0. 25, 0. 50, 0.75 and 1.0 mmol/L, the open probabilities of the channel were 0. 013±0. 002, 0. 082±0. 011, 0. 131±0. 012, 0.211 ± 0. 010 and 0. 062± 0. 009（P〈0. 01）, respectively. It appeared concentration-dependent within 0.75 mmol/ L batroxobin. In Ca^2＋ free-bath solution of cell-attach mode, Vp＋50 mV, when the concentrations of batroxobin were 0. 15, 0. 40, 0.60 and 1.0 mmol/L, the open probabilities of the channel were 0. 013±0. 001,0. 112±0. 007, 0. 193±0. 010 and 0. 301±0. 009（P〈0.05）, respectively. In the 6 cases of inside-out mode patch clamp, Vp ＋40 mV, when the concentrations of batroxobin were 0, 0. 25 and 0. 50 mmol/L, the open probabilities of the channel were 0. 012 ± 0. 007,0. 011 ± 0. 009 and 0. 013± 0. 008 （P〉0. 05）, respectively. There was no significant difference in open probabilities, average open/close times and amplitudes at different intraeellular batroxobin concentrations. Conclusion Batroxobin can affect the activation of the Kea channel through regulating the concentration of cytoplasmic free Ca^2＋. It may have a protective effect on neurons,

关 键 词：神经元 钙激活钾通道 巴曲酶 膜片钳 

分 类 号：R96[医药卫生—药理学]