人类羊膜细胞表达神经细胞和脂肪细胞表型  

Human Amnion Cells Express the Phenotypes of Neural Cells and Adipocytes in vitro

作　　者：郑佳坤[1] 杨立业[1] 许曼丹[1] 陈强[1] 李文玉[1] 汪朝阳[1] 

机构地区：[1]广东省潮州市中心医院神经外科,潮州521021

出　　处：《四川大学学报（医学版）》2006年第5期696-699,共4页Journal of Sichuan University(Medical Sciences)

基　　金：广东省自然科学基金(批准号04007929;012452;020001)资助

摘　　要：目的探索羊膜组织来源的细胞向神经细胞分化的可能性,为神经移植探寻新的细胞来源。方法采用组织块培养法培养羊膜细胞,酶消化法对细胞进行传代,应用免疫细胞化学的方法对细胞进行鉴定。结果从羊膜组织成功培养出细胞,细胞贴壁生长,在体外短期内可以稳定增殖和传代,传代细胞在神经干细胞的培养基中可以形成典型的球状结构,类似神经干细胞的神经球。细胞表达波形蛋白和巢蛋白两种神经干细胞的标志物,也表达神经元的标志物神经元特异性烯醇化酶和β管蛋白;大部分细胞表达多巴胺能神经元的标志物酪氨酸羟化酶。少数细胞表达星型胶质细胞的标志物胶质纤维酸性蛋白。部分细胞可以随机分化为平滑肌细胞,表达平滑肌肌动蛋白;在诱导条件下,羊膜细胞也可以分化为脂肪细胞。结论羊膜组织来源的细胞可以表达神经细胞的标志物,能够分化为脂肪细胞和平滑肌细胞,这种细胞对于神经移植有潜在的应用前景。Objective To search for culture conditions in which the cells from human amnion could differentiate into neural cells and hence to explore a new cell source for neural transplantation. Methods Amnion cells from human were cultured with tissue piece method, passaged by trypsin digestion and identified with immunoeytoehemistry. Results Amnion cells migrated from explants and primary culture was established; they could multiply and expand steadily in a short time, and they could be passaged by trypsin digestion. When cultured in serum-free neural stem cell media, these cells could form the same spheroid shape as the neurospheres of neural stem cells. They could express alpha smooth muscle aetin and differentiate into smooth muscle cells spontaneously, and could express nestin and vimentin, the markers for neural progenitors. Moreover, they could be stained by anti-βⅢ-tubulin, anti-neurofilament 200 and anti-NSE; the majorities could be stained by anti- tyrosine hydroxylase, the marker for dopaminergie neurons. Lower than 0. 1 % of the total cells were stained by GFAP, indicating the existence of astroeytes. The amnion cells could also differentiate into adipoeytes under specific induction. Conclusion Amnion cells could differentiate into adipocytes and smooth muscle cells; they could express the protein for neural cells; thus may represent an alternative stem cell source for CNS cell transplantation.

关 键 词：羊膜 于细胞 神经元 脂肪细胞 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]