WTK1细胞tk基因突变的多重PCR分析  

The tk Gene Mutation Analysis in WTK1 Cell Line by Multiplex PCR

作　　者：王怡净[1] 帅培强[1] 张立实[1] 

机构地区：[1]四川大学华西公共卫生学院营养与食品卫生学教研室,成都610041

出　　处：《四川大学学报（医学版）》2006年第5期790-793,共4页Journal of Sichuan University(Medical Sciences)

基　　金：国家自然科学基金(批准号30471473);博士点基金(批准号20020610036)资助

摘　　要：目的探讨WTK1细胞tk位点自发和诱发突变体的突变谱和突变热点。方法用甲磺酸甲酯、丝裂霉素C和叠氮钠染毒WTK1细胞后,筛选出tk基因突变集落,用多重PCR方法对WTK1细胞tk基因突变谱进行分析。结果三种受试物均能诱导WTK1细胞tk位点突变频率增高,且存在剂量-反应关系;所分析的突变体中绝大部分缺失tk基因外显子4和5-7。结论三种受试物对WTK1细胞均有致突变作用;tk位点的突变存在明显的突变热点。Objective To explore the tk gene mutation spectrum in spontaneous and induced WTK1 mutants. Methods After exposure to methyl methanesulfonate （MMS）, mitomycin （MMC） or sodiam azide （NAN3）, spontaneously-arising and induced mutants of WTK1 cell line were selected. The spectrum and hotspot of tk gene mutation were analyzed by multiplex PCR. Results Three chemicals used in this study induced tk mutation frequency （MF） to increase significantly in a close-dependent manner ; most of the analyzed mutants bad lost exon 4 and exon 5-7. Conclusion Three chemicals have mutagenic effect on WTK1 cell line, and obvious hotspot exists in tk gene mutation.

关 键 词：多重PCR WTK1细胞 TK基因突变 

分 类 号：R346[医药卫生—基础医学]