腹腔巨噬细胞CD54和TNF-α mRNA表达与妊娠期高血压疾病关系的研究  

Correlation of the expression of intercellular adhesion molecule CD54 and TNF-α mRNA in peritoneal macrophages with hypertensive disorders complicating pregnancy.

作　　者：赵彩珍[1] 乔福元[2] 

机构地区：[1]武警湖北总队医院妇产科,武汉430061 [2]华中科技大学同济医学院附属同济医院妇产科

出　　处：《现代妇产科进展》2006年第8期586-588,共3页Progress in Obstetrics and Gynecology

摘　　要：目的研究妊娠期高血压疾病患者腹腔巨噬细胞CD54和TNF-αmRNA的表达变化,探讨它们在妊娠期高血压疾病发病机理中的作用。方法体外培养30例妊娠期高血压疾病患者和10例正常孕妇腹腔液中的巨噬细胞,分别用流式细胞术半定量和逆转录多聚酶链反应(RT-PCR)方法测定实验组及对照组腹腔巨噬细胞表面CD54+及细胞内TNF-αmRNA的表达。结果轻度子痫前期、重度子痫前期组患者腹腔巨噬细胞CD54+细胞百分率较正常对照组明显增高,差异均有显著性(P<0.05);妊娠期高血压疾病组腹腔巨噬细胞TNF-αmRNA表达水平均明显高于对照组(P<0.05);相关性分析显示,CD54表达量与TNF-αmRNA含量呈正相关。结论在妊娠期高血压疾病中,巨噬细胞高表达CD54与TNF-αmRNA,二者表达量呈正相关;CD54与TNF-α可能是参与妊娠期高血压疾病的重要因子。Objective：To study the expression of CD54 and tumor necrosis factor-alpha （TNF-α） mRNA in peritoneal macrophages of hypertension disorder complicating pregnancy （HDCP） and the potential role of these cytokines. Methods：Peritoneal macrophages were obtained from 30 patients with HDCP and 10 normal pregnants. CD54 was measured by flow cytometry. Reverse transcription-polymerase chain reaction（RT-PCR） was used to detect TNF-α mRNA. Results： The percentage of CD54 positive cells in mild preclampsia and severe preclampsia groups was significantly higher than that in control （ P 〈 0.05 ）. The levels of TNF-α mRNA in peritoneal macrophages of HDCP group were significantly higher than that in control group（P 〈0.05）. There was significantly positive correlations between the expression of CD54 and TNF-α mRNA in peritoneal macrophages. Conclusion：The expression of CD54 and TNF-α mRNA in peritoneal macrophages of hypertensive patients were up-regulated, CD54 and TNF-α may be important factors in the pathogenesis of HDCP.

关 键 词：高血压 巨噬细胞 CD54抗原 肿瘤坏死因子Α 

分 类 号：R714.244[医药卫生—妇产科学]