急性缺氧/复氧时缺氧诱导因子-2α在妊娠期肝内胆汁淤积症患者胎盘中的变化  

Study of hypoxia-inducible factors in the placenta with intrahepatic cholestasis of pregnancy under acute hypoxic/nonhypoxic conditions.

作　　者：胡雅毅[1] 刘淑芸[2] 刘冀成[3] 

机构地区：[1]四川大学华西第二医院康复科,成都610041 [2]四川大学华西第二医院妇产科,成都610041 [3]成都信息工程学院

出　　处：《现代妇产科进展》2006年第8期608-610,F0003,共4页Progress in Obstetrics and Gynecology

基　　金：国家博士点基金资助(20020610030)

摘　　要：目的探讨缺氧诱导因子-2α(hypoxia-inducibletranscriptionfactors-2α,HIF-2α)在ICP患者胎盘上的表达及意义。方法通过急性缺氧/复氧体外培养胎盘绒毛组织,采用免疫组织化学染色技术,研究了ICP患者和正常晚孕妇女胎盘上HIF-2α蛋白质表达的变化。结果(1)常氧下(21%O2)ICP患者胎盘组织HIF-2α的表达明显高于正常晚孕组(P<0.01);(2)2%O2低氧浓度下培养4h后两组胎盘上HIF-2α的表达无显著性差异(P>0.05);正常组HIF-2α的表达较常氧下显著升高(P<0.01);(3)复氧15min,正常组胎盘绒毛HIF-2α的表达较缺氧4h显著降低(P<0.01)。而缺氧/复氧过程中ICP组HIF-2α的变化差异无统计学意义(P>0.05)。结论急性缺氧时ICP胎盘上HIF-2α的功能障碍可能导致不良妊娠结局。Objectives： As a key transcriptional regulator of downstream genes, hypoxiainducible factor （HIF） is a basic helix-loop-helix transcription factor which transactivates downstream molecules responses to hypoxia. Therefore, we would investigate the expression of the HIF in placenta of pregnant women with ICP. Methods：Protein from villous explant culture under hypoxia condition was probed for HIF-2α by immuno-histochemistry methods. Results： （ 1 ） Under normal concentration oxygen, HIF-2α was highly expressed in the placentae of pregnant women with ICP （P 〈 0. 01 ） ;（2）When the placental villous tissues cultured for 4h under hypoxic condition （2% O2 ）, the HIF-2α expression in the placentae of pregnant women with ICP was normal compared to those normal term placentae;the expression of HIF-2α was highly increased under hypoxia condition than that under normal concentration oxygen;（3 ）With the oxygen concentration recovered ,the high oxygen concentration （21% O2 ） decreased the expression HIF-2α in 15 minutes（ P 〈O. 05 ） in normal group. Respectively, no significant difference was revealed in the change of the HIF-2α in ICP （P 〉0.05 ）. Conclusion：This study suggested that under hypoxic conditions the abnormal change of HIF-2α may play a role in bad pregnancy outcome in ICP.

关 键 词：妊娠期肝内胆汁淤积症 缺氧诱导因子-2Α 

分 类 号：R714.56[医药卫生—妇产科学]