反复热性惊厥大鼠体内皮质醇水平变化  

Change of Cortisol Level in Rats with Frequent Febrile Seizures

作　　者：郑飞霞[1] 李光乾[1] 

机构地区：[1]温州医学院附属育英儿童医院神经内科,浙江温州325027

出　　处：《实用儿科临床杂志》2006年第18期1250-1251,1266,共3页Journal of Applied Clinical Pediatrics

基　　金：温州市科技局科技发展计划项目资助(Y2004A012)

摘　　要：目的探讨反复热性惊厥(FS)大鼠体内糖皮质激素水平的变化。方法将大鼠随机分为正常对照组(n=10,NC组),高热对照组(n=12,HC组)和热性惊厥组(n=21,FS组)。用ELISA方法测大鼠海马匀浆和血清皮质醇水平;电镜观察大鼠海马CA1区神经元超微结构变化。结果1.FS组大鼠海马CA1区出现部分神经元肿胀,线粒体嵴模糊或消失;亦有表现为核固缩。2.FS组大鼠血清、海马皮质醇水平[分别为(26.57±15.94)μg/L,(4.11±1.74)μg/L]明显低于HC组[分别为(40.04±15.72)μg/L,(6.70±3.45)μg/L](P均<0.05)。HC组大鼠血清皮质醇水平明显高于NC组[(22.74±12.97)μg/L](P<0.05)。FS组大鼠血清、海马皮质醇水平与NC组比较无显著差异(P均>0.05)。血清和海马皮质醇水平间存在正相关(r=0.40 P<0.05)。结论反复FS可抑制发热刺激的皮质醇分泌;皮质醇未参与反复FS导致的脑损伤过程。Objective To evaluate cortisol level in experimental frequent febrile seizures model rats. Methods Rats were divided into 3 groups： normal control group （ n = 10, NC group ）, hyperthermic control group （n = 12, HC group） and febrile seizures group （ n = 21, FS group）. Cortisol level in serum and hippocampus of rats were determined by ELISA. Ultrastructural changes of neurons in hippocampal region CAI was observed by electron microseop. Results 1. No special ultrastructual findings were observed from neurons in hippocampal CAI in NC and HC group. In FS group, the neuronal degeneration and edema, the mitochondrial degeneration and edema, the mitochondrion ridge obscured or disappeared. 2. Cortisol levels in serum and hippocampus in FS group [（26.57 ± 15.94）μg/L and （4.11 ± 1.74）μg/L, respectively] were significantly lower than those in HC group [ （40.04 ± 15.72）μg/L and （6.70 ± 3.45）/μg/L, respectively]（P〈0.05）. Serum cortisol level in HC group was significantly higher than that of NC group[（22.74 ±12.97）μg/L] （P 〈 0.05 ）. There were no significant difference in serum and hippocampus cortisol level between FS group and NC group（ P 〉0.05 ）. Positive correlations were demonstrated between serum cortisol level and hippocompus cortisol level（ r = 0.40 P 〈 0.05 ）. Conclusions Frequent FS can cause the decrease of cortisol level which increased by heat stress. Cortisol may not play a role in pathology of FS.

关 键 词：惊厥 发热性 皮质醇 大鼠 

分 类 号：R720.597[医药卫生—急诊医学]