液氮冻存降低成骨细胞免疫原性的实验研究  

Experimental Study of Fluid Nitrogen Cryopreservation on the Reducing of Osteoblasts Immunogenicity

作　　者：于洪波[1] 魏奉才[2] 孙善珍[3] 

机构地区：[1]山东大学口腔医院口腔颌面外科,山东济南250012 [2]山东大学齐鲁医院口腔科,山东济南250012 [3]山东大学口腔医院病理科,山东济南250012

出　　处：《口腔颌面外科杂志》2006年第3期215-218,共4页Journal of Oral and Maxillofacial Surgery

基　　金：山东省卫生厅资助项目(CAICJA17)

摘　　要：目的:探讨液氮冷冻(fluid nitrogen cryopreservation)对成骨细胞免疫原性的影响。方法:体外培养成骨细胞,传代鉴定后液氮冻存3个月,复苏。利用流式细胞仪(flow cytometry,FCM),通过间接免疫荧光技术测定冻存前后成骨细胞的免疫原性。同时采用成骨细胞、淋巴细胞混合培养(osteoblasts and lymphocytes mixed culture,OLMC)的方法,测定液氮冷冻对成骨细胞免疫原性的影响。结果:体外培养细胞经鉴定为成骨细胞,液氮冻存3个月后存活率>90%。FCM及OLMC实验结果均表明冻存后成骨细胞的免疫原性显著降低(P<0.01)。结论:低温液氮冷冻是一种理想的降低成骨细胞免疫原性的方法。Objective： To investigate the effects of fluid nitrogen cryopreservation on the immunogenicity of osteoblasts. Methods： The osteoblasts cultured and identified by alkaline phosphatase（ALP） stains and calvaria stains in vitro, were cryopreservated by fluid nitrogen for 3 months and then thawed. Having been labelled by immunofluorescene technique, the MHC-1 antigen of osteoblasts was examined by flow cytometry assy （FCM）. At the same time, the immunogenicity of osteoblasts were examined by establishing osteoblasts and lymphocytes mixed culture model（OLMC）. Results： Cells cul- tured in vitro were identified as osteoblasts. The results of both FCM and OLMC experiments showed immunogenicity of osteoblasts was significantly reduced by fluid nitrogen cryopreservation （P〈0.01）. Conclusion： Fluid nitrogen cryopreservation is an ideal method to reduce the immunogenicity of osteoblasts.

关 键 词：液氮冷冻 成骨细胞 免疫原性 

分 类 号：R392[医药卫生—免疫学]