电化学发光法在检测孕产妇乙肝中的应用  

Application of HBsAg detected by electrochemiluminescence assay in pregnant women

作　　者：李化会[1] 李化英 莫晓媚[3] 王青[1] 李莉[1] 侯蔚[1] 纪秀兰[1] 宋晓英[1] 纪国欣[4] 

机构地区：[1]青岛大学医学院附属市立医院检验科,青岛266071 [2]山东省新泰市泉沟镇卫生院,新泰271207 [3]青岛市妇女儿童医疗保健中心药剂科,青岛266011 [4]青岛大学医学院附属市立医院产科,青岛266071

出　　处：《现代生物医学进展》2006年第9期59-60,共2页Progress in Modern Biomedicine

摘　　要：背景与目的：探讨电化学发光法(electrochemiluminescence ECL)在检测孕产妇乙肝病毒中的应用价值,从而正确指导乙肝孕产妇产后的喂养方式。方法：用ELISA法和ECL法平行测定孕产妇血清乙肝病毒血清标志物(HBV-M),并对ELISA法检测表面抗原为阴性而ECL法为低浓度的标本,用荧光定量PCR测定病毒DNA。结果：ECL法表面抗原的检出率(9．66%)明显高于ELISA法(6．54%),p＜0．05,ELISA法检测为阴性而ECL法为低浓度的标本,病毒DNA均为阳性。结论：ECL法测定HVV-M的敏感性比ELISA法高,可以更灵敏的检出低浓度的表面抗原,从而可以更好的指导孕产妇产后的喂养方式,但HBcAb阳性率过高。Objective： To investigate the clinical value of HBV detection by the method of ECL in pregnant women and direct them how to feed their children. Methods： HBV markers in serum were detected by ELISA and ECL respectively. We used FQ- PCR to detect HBV - DNA in those samples whose HBsAg were negative by ELISA, but were a little higher than that of normal women by ECL. Results： The rate of positive HBsAg by ECL and ELISA are 9.66%, 6.54% respectively. The difference between them is significant（p 〈 0.05）. HBV - DNA is positive in those samples whose HBsAg are a little higher by the method of ECL. Conclusion： The sensitivity of HBV markers detection by ECL is higher than ELISA, so we can direct pregnant women more correctly. Unfortunately, the positive rate of HBcAb by ECL is much higher.

关 键 词：电化学发光免疫分析法 乙型肝炎病毒 孕产妇 

分 类 号：R446[医药卫生—诊断学]