反相高效液相色谱法测定大鼠头孢氨苄血药浓度  

Determination of Cefalexin concentration in pat plasma by reversed phase high performance liquid chromatography

作　　者：罗楠[1] 刘放南[1] 万秀珍[1] 谭力[1] 

机构地区：[1]南京军区南京总医院解放军普通外科研究所,江苏南京210002

出　　处：《医学研究生学报》2006年第9期783-784,787,共3页Journal of Medical Postgraduates

基　　金：南京军区南京总医院科研基金资助项目(批准号:2005049)

摘　　要：目的：高效液相色谱法测定大鼠血浆中头孢氨苄浓度。方法：血浆样品用超滤法去除蛋白质直接进样。以μBondapak^TM C18柱（4．6mm×250mm，5μm）为色谱柱，水-甲醇（400：100，v／v，用1．0mol／L醋酸调pH至4．0）为流动相，流速为0．8ml／min，柱温25℃；二极管阵列检测波长为254nm。结果：头孢氨苄浓度在0．63～10．0μg／ml范同内线性关系良好，相关系数〉0．9999；其日内和日间相对标准差（RSD）〈10％（n=5），方法回收率为98．1％～100．1％（n=5）。结论：该法快速简便，适用于临床血药浓度研究和间接评价结肠上皮二肽载体（PepT1）的功能。Objective ： A high performance liquid chromatographic method for the determination of Cefalexin in rat plasma was developed. Methods： The plasma sample was deproteinized by an uhrafihration. A reversal phase （ μBondapak^TM 4.6 mm ×250 mm, 5 μm） column was used with a mobile phase of water-methanol （400 ： 100, v/v ; pH 4.0） and flow rate of 0.8 ml/min. The photodiode array detector was used. Results： A good linearity was obtained from 0.63 - 10.0 μg/ml of Cefalexin, the relation coefficient was more than 0. 999 9. The relative standard derivations for within-day and between-day were all 〈 10% （ n = 5 ）, and the recoveries were 98.1% - 100.1% （ n = 5 ）. The average recoveries of method （ n = 5 ） was 98.1%. Conclusion ： This method was simple and rapid, and was suited for clinical investigation of Cefalexin in rat plasma.

关 键 词：头孢氨苄 大鼠 反相高效液相色谱法 

分 类 号：R969.1[医药卫生—药理学]