时间因素对电离辐射后AT细胞hTERT mRNA表达的影响  

Effects of Time Factor After Exposed to Ionizing Radiation on hTERT mRNA Expression in AT Cell Line

作　　者：盛方军[1] 曹建平[2] 朱巍[2] 朱财英[2] 冯爽[2] 宋建元 李翀[2] 樊赛军 

机构地区：[1]浙江省宁波市第二医院放疗科 [2]苏州大学放射医学与公共卫生学院 [3]Department of Oncology Lombardi Comprehensive Cancer Center Georgetown University Washington DC20057USA

出　　处：《苏州大学学报（医学版）》2006年第4期535-538,共4页Suzhou University Journal of Medical Science

基　　金：国家自然科学基金资助项目(30170288);江苏省高校自然科学基金重点资助项目(04KJA180121);江苏省研究生创新计划(Xm04-67);苏州大学医学发展基金重点资助项目(EE126032)

摘　　要：目的研究时间因素对电离辐射后共济失调性毛细血管扩张(ataxia telangiectasia,AT)综合征患者皮肤的成纤维细胞系AT细胞(AT5BIVA)hTERT mRNA表达的影响。方法以源于正常人皮肤的成纤维细胞系GM细胞(GM0639)为对照,细胞经5 Gy(剂量率1.0 Gy/min)60Coγ射线照射后继续培养2、24、48、72 h,应用RT-PCR法,观察AT细胞、ATM+-AT细胞和GM细胞的hTERT mRNA表达的变化。结果照射后细胞hTERTmRNA的表达随培养时间的增加而增加;AT细胞hTERT mRNA的相对表达量高于ATM+-AT细胞和GM细胞(P<0.05),后两者无显著性差异(P>0.05)。结论电离辐射能诱导细胞hTERT mRNA的持续表达;ATM能下调AT细胞hTERT mRNA的表达。Objective To study the effects of time factor after exposed to ionizing radiation on expression of hTERT mRNA of fibroblast cells（AT5BIVA cells） from the skin of the ataxia telangiectasia（AT） patients. Methods Using normal GM0639 cells as the control, the variance of expression of hTERT mRNA of AT,ATM^＋-AT and GM cells after exposed to 5 Gy 60C0 γ-rays was observed at the 2nd,24th,48th and 72th hour by RT-PCR. Results After exposed to 5 Gy 60Co γ-rays, expression of hTERT mRNA in cells increased in line with the increase in culture time. The relative expression of hTERT mRNA in AT cells was higher than that in ATM^＋ -AT and GM cells（P 〈 0.05）. There was no significant difference in relative expression of hTERT mRNA in ATM^＋ -AT cells and than that in GM cells（P 〉0.05）. Conclusion Ionizing radiation can induce continuous expression of hTERT mRNA in cells. ATM can down-regulate expression of hTERT mRNA in AT cells.

关 键 词：AT细胞 ATM 电离辐射 端粒酶逆转录酶 

分 类 号：R811.5[医药卫生—放射医学]