阿克拉霉素A与DNA作用的光谱学研究  

Studies on Aclacinomycin Spectral Characters and the Interaction between Aclacinomycin and DNA by Spectroscopic Method

作　　者：胡敏[1] 张镇西[1] 沈国励[2] 刘娅莉[2] 

机构地区：[1]西安交通大学生物医学信息工程教育部重点实验室 [2]湖南大学化学化工学院

出　　处：《光谱学与光谱分析》2006年第9期1668-1671,共4页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金(20205005;20205004);西安交通大学自然科学基金资助项目

摘　　要：文章研究了阿克拉霉素A（ACR）吸收光谱和荧光光谱特性及其与DNA的相互作用。研究结果表明，ACR荧光光谱和吸收光谱受pH值影响较大，且随DNA加入产生减色效应。通过计算，ACR与DNA的结合常数比单纯以嵌入式与DNA结合的葸醌类化合物大约1～2个数量级为2．7×10^6mol·L^-1，结合位点数为0．67碱基对，ACR与DNA结合方式复杂，ACR为非经典嵌入剂。The interaction of aclacinomycin（ACR） and DNA was investigated by fluorescence spectrum, and the charactcristics of the fluorescence and absorption of aclacinomyein （ACR） were studied. The results indicate that there are two situations： in the case that the concentration ratio of ACR to DNA is small, the ACR is intercalated into the stacked base pairs of DNA; in the other case that the concentration ratio of ACR to DNA is great, the interaction between ACR and DNA is complex. The binding constant of the interaction between ACR and DNA, calculated by the fluorescence titration method, is 2.7 × 10^6 mol·L^-1, and the binding site number is about 0.67 base pairs.

关 键 词：阿克拉霉素A DNA 荧光光谱 

分 类 号：O657.3[理学—分析化学]