微量量热法研究黄连素对细菌的代谢作用  

Study on the Bacteriostasic Activity of Berberine by Microcalorimetry

作　　者：王建涛[1] 闫咏梅[1] 高庆柱 孔哲[1] 高桂兰[1] 郁雪梅[1] 张洪林[1] 

机构地区：[1]曲阜师范大学化学科学学院,273165 [2]王庄乡冯村中学,山东省曲阜市273129

出　　处：《曲阜师范大学学报（自然科学版）》2006年第4期99-103,共5页Journal of Qufu Normal University(Natural Science)

摘　　要：从黄连中提取黄连素并用熔点法、红外光谱法、薄层色谱法、液相色谱—质谱联用法对其进行了表征.用瑞典产的2277型热活性检测仪测定了在310 K时不同浓度的黄连素溶液对大肠杆菌、金黄色葡萄球菌、枯草杆菌代谢作用的热功率—时间曲线,计算出细菌生长的速率常数,建立了速率常数与药物浓度之间的关系,进而确定了黄连素对3种细菌的最佳用药浓度.In this paper, Berberine is extracted from Coptis and identified by mensuration of melting point, infrared spectral identification, thin-layer chromatography, high efficiency liquid chromatographymass spectrometry. Using 2277 Thermal Activity Monitor（Sweden）, the power- time curves of different concentrations of Berberine solution for E. coil, S. aureus and B. subtilis are determined, the growth rate constant （μ）of different concentrations（c） are calculated at 310 K,the relationship of μ-c is established and the optimum allowable concentration is obtained.

关 键 词：微量量热法 黄连 小檗碱 大肠杆菌 金黄色葡萄球菌 枯草杆菌 生长速率常数 

分 类 号：O642.3[理学—物理化学]