应用cDNA-AFLP研究甘蓝型油菜显性细胞核雄性不育差异表达基因  

Differentially Distinguished Expressed Genes in Dominant Genic Male Sterility (DGMS) Brassica napus Using cDNA-AFLP

作　　者：吴建勇[1] 沈俊儒[1] 刘平武[1] 杨光圣[1] 

机构地区：[1]华中农业大学作物遗传改良国家重点实验室,武汉430070

出　　处：《中国农业科学》2006年第9期1921-1926,共6页Scientia Agricultura Sinica

基　　金：国家"973"计划项目(2001CB108807);国家自然科学基金(30080012);教育部博士点基金(20010504002);长江学者和创新团队发展计划(IRT0442)资助

摘　　要：【目的】显性细胞核雄性不育(DGMS)是油菜杂种优势利用的重要途径。为了更好地理解该不育系统的分子机理,对甘蓝型油菜显性核不育的育性相关基因进行初步的研究。【方法】利用cDNA-AFLP对显性核不育材料两用系Rs1046AB的不育株和可育株进行了对比分析。【结果】共得到32个差异片段,分别属于27个uniGene,其中在可育株中增强或特异表达的有26个,不育株中只有1个。17个可以在NCBI数据库中找到同源序列(包括EST),10个没有找到同源序列。对17个已知序列的分析表明,这些片段参与了代谢、转录、细胞周期、蛋白质修饰、细胞间运输、信号转导和花发育等相关过程。此外,Northern印迹分析结果表明检测片段的表达模式与PAGE胶结果一致。【结论】本研究为更深入的研究甘蓝型油菜显性核不育的育性相关基因提供了基础,并对雄配子的发育研究也具有一定参考价值。[Objective] Dominant genic male sterility （DGMS） is an important way to utilize the heterosis ofBrassica napus. So, pilot studies were conducted for better understanding the mechanism of the DGMS. [Method] By using cDNA-AFLP, the fertile plants and sterile plants between the homozygous DGMS two-type line Rs1046AB were studied. [Result] There were 32 differentially expressed bands between them which belonged to 27 uniGene. Among them, 26 bands were only expressed or expressed in a higher level in fertile plants, and only 1 band in sterile plants. 17 bands could find homologous sequence, but the other 10 bands were maybe some novel genes. The 17 homologous sequences participated in the following biological process： metabolism, transcription, cell cycle, protein fate, signal transduction mechanism, cellular transport and flower development etc. Furthermore Northern blot demonstrates that it was consistent with the result of PAGE. [Conclusion] This research could give basic information about the dominant genic male sterility in Brassica napus and male gametogenesis.

关 键 词：甘蓝型油菜 显性细胞核雄性不育 CDNA-AFLP 雄配子发育 

分 类 号：S565.4[农业科学—作物学]