顶空气相色谱法测定克拉霉素中的丙酮溶剂残留  

Determination of Solvent Residue of Acetone in Clarithromycin by Headspace Gas Chromatography

作　　者：杨雪蕊[1] 梁振益[2] 张名楠[1] 

机构地区：[1]海南大学公共实验中心 [2]海南大学理工学院,海南海口570228

出　　处：《海南大学学报（自然科学版）》2006年第3期243-246,共4页Natural Science Journal of Hainan University

摘　　要：建立了克拉霉素中丙酮溶剂残留量的测定方法．采用顶空气相色谱法，氢火焰离子化检测器（FID），以二甲基甲酰胺（DMF）为溶剂，HP—FFAP弹性石英毛细管柱为色谱柱（30m×0.25mm，0.25μm），氮气为载气，分流比为1：60，进样口温度为200℃，检测器温度250℃，柱温采用程序升温、丙酮的质量浓度在0．002～4g·L^-1的范围内呈良好的线性关系（r=0．9995），回收率为99．2％（RSD=1．9％）检出限小于2．0mg·L^-1．A method for the determination of residue of acetone solvent was established using laeadspace gas chromatography. The solvent used in this determination was DMF, the column used was HP-FFAP（ 30m ×0.25mm, 0. 25μm） , the carrier gas used was nitrogen with split ratio of 1：60, the injector temperature was 200℃, the detector temperature was 250℃ , and the column temperature was programmed in this determination process. Acetone showed a good linear relationship in the range of 0. 002-4 g · L^- 1 in sample solution （ r = 0. 9995 ）, recovery rate was 99.2% （ RSD = 1.9% ）, and detecting limit was less than 2.0 mg · L^-1.

关 键 词：顶空气相色谱法 克拉霉素 溶剂残留 丙酮 

分 类 号：O657.7[理学—分析化学]