当归A_3活性部位的抗炎作用及其对大鼠离体子宫环氧化酶-2表达的影响  

Effect of angelica A_3 active fraction on anti-inflammation and cyclooxygenase-2 expression of isolated rat uterus

作　　者：沈建芬[1] 肖军花[1] 王嘉陵[1] 

机构地区：[1]华中科技大学同济医学院药理学系,湖北武汉430030

出　　处：《中草药》2006年第9期1371-1374,共4页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(30171095)

摘　　要：目的研究当归A3活性部位（A3）的抗炎作用及其对脂多糖（LPS）诱导离体大鼠子宫环氧化酶-2（Cox-2）表达增高的影响。方法采用二甲苯致小鼠耳廓肿胀和角叉菜胶致大鼠足趾肿胀法进行抗炎药效学实验；采用RT—PCR和Western blotting法检测Cox-2mRNA及蛋白表达水平。结果A3（1、5、10mg／kg）可剂量依赖性地抑制二甲苯所致的小鼠耳廓肿胀和角叉菜胶所致的大鼠足趾肿胀。LPS1μg／mL可显著增加离体大鼠子宫Cox-2mRNA和蛋白表达水平。A。（10～320mg／L）剂量依赖性地抑制LPS诱导的子宫Cox-2mRNA和蛋白表达水平增高。结论A3具有较强的抗炎作用，其抗炎作用机制可能与抑制Cox-2mRNA及蛋白表达有关。Objective To study the inhibitory effects of angelica A3 active fraction （A3） inflammation and up-regulation of eyelooxygenase-2 （Cox-2） expression of rat uterus induced by lipopolysaeeharides （LPS）. Methods The anti-inflammatory effects of A3 were investigated in rats using the earrageenininduced paw swelling model and in mice using dimethylbenzene-indueed ear edema model; RT-PCR and Western blotting were used to analyze Cox-2 mRNA and the protein expression levels. Results A3（1, 5, and 10 mg/kg） dose-dependently inhibited dimethylbenzene-indueed ear edema in mice and paw swelling in rats by ig administration. LPS 1μg/mL could significantly increase the level of Cox-2 mRNA and protein expression. A3 （10-320 mg/L） could concentration-dependently inhibit Cox-2 mRNA and protein overexpression stimulated by LPS. Conclusion A3 possesses better anti-inflammatory effects than angelica oil, which maybe relates to its inhibitory effects on Cox-2 overexpression.

关 键 词：当归 A3活性部位 抗炎作用 环氧化酶-2 

分 类 号：R285.5[医药卫生—中药学]