组织型纤溶酶原激活剂促进小鼠坐骨神经血-神经屏障损伤后的修复  

Recovery of blood-nerve barrier is delayed in tissue plasminogen activator gene knockout mice after sciatic nerve crush

作　　者：凌长春[1] 陶贤梅[1] 萧瑶[1] 胡华[1] 陈祖林[1] 宋后燕[1] 

机构地区：[1]复旦大学上海医学院分子医学教育部重点实验室,上海200032

出　　处：《复旦学报（医学版）》2006年第5期597-600,共4页Fudan University Journal of Medical Sciences

基　　金：21世纪教育振新计划;十五"211"工程;上海市科委(02DJ14005)资助

摘　　要：目的观察组织型纤溶酶原激活剂(tissue plasminogen activator,t-PA)对坐骨神经血-神经屏障损伤后修复的影响。方法选取野生型小鼠和t-PA基因敲除纯合子小鼠,利用持针器钳夹坐骨神经20 s。在损伤后第3,7,11,15天观察小鼠坐骨神经血-神经屏障修复情况。采用尾静脉注射Evans Blue,观察野生型小鼠组和t-PA基因敲除小鼠组血-神经屏障渗透性的差异。利用免疫荧光和Western Blot观察两组小鼠损伤段坐骨神经血-神经屏障重要的组成性蛋白occludin的恢复情况。结果小鼠坐骨神经损伤后,t-PA基因敲除小鼠组血-神经屏障的通透性在损伤后第7,11,15天高于野生型小鼠组。免疫荧光及其Western Blot显示t-PA基因敲除延缓了坐骨神经损伤后occludin的表达。结论t-PA基因缺失对小鼠坐骨神经血-神经屏障损伤后的修复有一定的阻碍作用,与坐骨神经血-神经屏障损伤后,其组成性蛋白occludin的表达减少有关。Purpose To observe the effect of tissue plasminogen activator （t-PA） on the recovery of blood-nerve barrier after sciatic nerve crush. Methods For crush injury, the sciatic nerve was placed in a 2-ram-wide needle holder and crushed for 20 seconds. The recovery of blood-nerve barrier was observed by intravenous injection of fluorescent Evans Blue on days 3, 7, 11 and 15 after crush. For the permeability of blood-nerve barrier, the fluorescent intensity was analyzed. The occludin was observed by immunofluorescence and Western blot. Results The leakage of Evans Blue into the connective tissue in the endoneurium was more evident in the sciatic nerve of t-PA gene knockout mice than the one of wild type mice on days 7, 11 and 15 after crush. The positive staining of occludin was decreased in the sciatic nerve of t-PA gene knockout mice compared with the one of wild type mice on days 7, 11 and 15 after crush. It was confirmed further by Western blot. Conelosions t-PA plays a positive role in the recovery of blood-nerve barrier and the recovery expression of occludin is delayed in the crushed sciatic nerve after disruption of t-PA gene.

关 键 词：组织型纤溶酶原激活剂基因敲除小鼠 坐骨神经损伤 血-神经屏障 紧密连接蛋白 

分 类 号：R322.85[医药卫生—人体解剖和组织胚胎学]