东方田鼠的同工酶与异构蛋白生化基因位点  

Loci of Several Isozymes and Proteins in Microtus fortis

作　　者：倪丽菊[1] 潘漪清[1] 谢建云[1] 高诚[1] 

机构地区：[1]上海市实验动物质量监督检验站

出　　处：《中国实验动物学报》2006年第3期186-192,共7页Acta Laboratorium Animalis Scientia Sinica

基　　金：上海市科技发展基金项目(编号:014909002)

摘　　要：目的观察四类东方田鼠在10个同工酶与异构蛋白生化基因位点上的基因分布特征，以研究它们间的遗传关系。方法应用乙酸纤维素膜电泳对四类东方田鼠的同工酶与异构蛋白生化基因位点进行测定分析。结果与结论黑龙江小体型东方田鼠与其他三类东方田鼠的电泳结果差异较大，在6个位点上都存在其所特有的等位基因，并且在其中的3个位点上它的基因型与其他三类鼠完全不同；湖南、宁夏和黑龙江大体型东方田鼠三者间的遗传距离在0．0633-0．2107之间，而黑龙江小体型东方田鼠与其他三类鼠的遗传距离在0．7068～0．8953之间；UPGMA聚类分析显示，湖南、宁夏和黑龙江大体型东方田鼠聚为一类，亲缘关系较近，而黑龙江小体型东方田鼠单独为一类，与其他三种鼠的亲缘关系均相对较远。Objective To analyze the phylogenetic relationship among four groups of Microtus fortis by gene distribution characteristics of ten biochemical loci. Method Ten isozymes and proteins of Microtus fortis were investigated by cellulose acetate membrane electrophoresis. Result and Conclusion The electrophoretic patterns of the small voles from Heilongjiang province revealed great divergence in comparison with the other three groups. The small voles from Heilongjiang had their particular alleles at six loci and the phenotypes of them were completely different at three of these loci. The genetic distances among the voles from Hunan, Ningxia and the big voles from Heilongjiang ranged from 0.0633 to 0. 2107, while that between the small voles from Heilongjiang and the other three groups ranged from 0.7068 to 0.8953. The phylogenetic tree constructed by UPGMA showed that the voles from Hunan, Ningxia and the big voles from Heilongjiang could be gathered into one group, but the small voles from Heilongjiang were in another group alone. Those results indicated that the phylogenetic relationship among the voles from Hunan, Ningxia and the big voles from Heilongjiang is closer than that between them and the small voles from Heilongjiang.

关 键 词：东方田鼠 电泳 醋酸纤维素 基因 遗传特性 

分 类 号：R332[医药卫生—人体生理学]