金属催化剂有效快速裂解SARS冠状病毒和其他微生物  

Rapid Inactivation of SARS Coronavirus and Other Microorganisms by Metal Catalysts

作　　者：韩俊[1] 陈岚[1] 段淑敏[1] 高晨[1] 张宝云[1] 贺红[2] 董小平[1] 

机构地区：[1]中国疾病预防控制中心病毒病预防控制所,北京100052 [2]中国科学院生态环境科学研究中心,北京100085

出　　处：《中国实验动物学报》2006年第3期209-212,共4页Acta Laboratorium Animalis Scientia Sinica

基　　金："863"项目(编号:2003AA208402和2003AA208201)

摘　　要：目的研究两种金属催化剂Ag/Al2O3和Cu／Al2O3对SARS冠状病毒和其他呼吸道疾病传播的阻断作用。方法100μL10^6 TCID50／mL SARS-CoV，100μL 10^6PFU／mL。表达重组仓鼠朊蛋白的杆状病毒和10^6大肠杆菌分别缓慢地滴加至两种圆形金属催化剂的表面，吸附5．20min。使用不同培养液洗涤金属催化剂表面，检测洗涤物的病毒感染性和细菌的增殖。结果病毒和细菌分别吸附5和20min后，SARS-CoV和杆状病毒的病毒滴度下降到非常低的水平，甚至难以检测到；大肠杆菌组经培养后无克隆生长。5min吸附后，杆状病毒表达的重组PrP的表达下降到21．8％；20min吸附后，没有PrP的表达。电镜检测发现大肠杆菌的细胞壁崩解，细胞破碎。结论与金属催化剂Ag/Al2O3和Cu／Al2O3表面接触后，破坏了SARS-CoV，杆状病毒和大肠杆菌的复制增殖能力。两种金属催化剂利用空气中的氧分子有效的杀灭吸附于其表面的病毒和细菌，有希望用来作为医院、家庭和公共场所的空气消毒。Objective The transmission of SARS coronavirus is believed to be through droplets and fomites, as well as poaaibly through alrbeme infection. Air disinfection may play an important role in interupting the transmission pathway for SARS and other respiratory infectious diseases. The aim of the present study was to investigate the possibility of application of two metal catalysts, Ag/Al2O3 and Cu/Al2O3 , to disrupt the transmission route of SARS eoronavirus. Methods Two metal catalysts Ag/ Al2O3 and Cu/Al2O3 were pressed into wafers. 100/μL 10^6 TCID50/mL SARS-CoV, 100/μL 10^6 PFU/mL recombinant baeulovirus expressing hamster＇ s PrP protein and roughly 10^6 E. coli were slowly dropped onto the surfaces of the catalyst wafers and exposed for 5 and 20 min, respectively. After eluted from the surfaces of wafers, the infectivity of viruses and propagation of bacteria were measured. The expression of PrP protein was determined by Western blot. The morphological changes of bacteria were examined by electron microscopy. Results After exposure on the catalysts surfaces for 5 and 20 min, the infectivity of SARS-CoV in Vero cells and baeulovirus in Sf9 cells dropped down to very low even undeteetable level, and no colony was detected in bacteriaculture. The expression of PrP protein reduced to 21,8% in the preparation exposed for 5 min and undetectable in that for 20 min. Bacterial membrane seemed to be disrupted and the cytoplasm seemed to be effluent from cell bodies. Conclusion Exposure of SARS-CoV, baculovirus and E. coli on the surface of Ag/Al2O3 and Cu/Al2O3 wafers are inactivated with loss of replication and propagation abilities of those microorganisms. The inactivation ability of metal catalysts needs to interact with and utilize molecular oxygen in the air.

关 键 词：金属催化剂 SARS冠状病毒 灭活 感染性 

分 类 号：R18[医药卫生—流行病学]