鸡胚内神经营养活性物质的提取及其生物活性  

Extracting Neurotrophic Active Substance from Chicken Embryo and Study of Their Bioactivity

作　　者：陈姝[1] 何蕴韶[1] 程钢[1] 高劲松[1] 

机构地区：[1]中山大学达安基因诊断中心,广东广州510080

出　　处：《中山大学学报（医学科学版）》2006年第5期481-485,共5页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：国家卫生部攻关计划基金资助项目(199983)

摘　　要：【目的】从鸡胚内提取具有神经营养生物活性的组分。【方法】分别取胚龄为E10、E16、E19的鸡胚,匀浆离心后,上清超滤,获得5个组分,即相对分子质量Mr<3×103、Mr=3×103～<10×103、Mr=10×103～<30×103、Mr=30×103～≤50×103与Mr>50×103。采用新生大鼠海马神经元与鸡胚背根节体外培养方法,观察各组分的促神经元存活及突起生长的作用。用MTT法测定神经元的活性。【结果】胚龄E16与E19的鸡胚内相对分子质量Mr>50×103的组分有明显的促进海马神经元细胞存活、分化和突起生长及鸡胚背根节突起生长的作用。【结论】鸡胚内含有较强的促进神经元存活、分化与突起生长的神经营养活性物质。[Objective] To extract neurotrophic active substance from chicken embryo and study their bioactivity. [Methods] Homogenate extracts were prepared from chicken embryos from embryonic day 10（E10）, E16 and E19.The extracts were ultrafiltrated and separated into 5 components of Mr 〈 3×10^3, Mr = 3×10^3～〈 10×10^3, Mr = 10×10^3～〈 30×10^3, Mr =30×10^3～≤ 50×10^3, and Mr 〉50×10^3. Two bioassay methods including chicken embryo dorsal root ganglia （DRG） culture and neonatal rat hippocampal neuron culture were applied to assay the effects of components on survival and neurite formation of neuron. The neuron- surviving activity was examined by MTT method. [Results] The component of Mr 〉50×10^3 from E16 and E19 could significantly enhance survival, differentiation, and neurite outgrowth of rat hippocampal neuron and could promote neurite - outgrowth on chicken embryo dorsal root ganglia （DRG）. [Conclusion] This finding suggests that neurotrophic active substances from chicken embryo possessed strong neurotrophin-like activity which could enhance survival, differentiation, and neurite outgrowth of neuron.

关 键 词：鸡胚 超滤 神经营养物质 海马神经元 背根节 神经突起生长 

分 类 号：R338.2[医药卫生—人体生理学]