重组人催乳素对大鼠子宫内膜异位模型作用的初步研究  

Effect of rhPRT on Endometriosis in Rat Model

作　　者：徐成康[1] 陈玉清[1] 谢梅清[2] 张凤丽[2] 

机构地区：[1]中山大学第一附属医院妇产科,广东广州510080 [2]中山大学第二附属医院妇产科,广东广州510080

出　　处：《中山大学学报（医学科学版）》2006年第5期506-509,共4页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：广东省科委攻关基金资助项目(C30602)

摘　　要：【目的】初步了解重组人催乳素(rhPRL)对子宫内膜异位症(EM)的治疗作用。【方法】先建立大鼠EM模型50只,将建模成功大鼠随机分为6组:实验1组10例,实验2组10例,实验3组10例,Danazol对照组10例,生理盐水对照组6例,空白对照组4例。然后用不同浓度的rhPRL(每日分别为200μg/kg、100μg/kg、50μg/kg)给实验大鼠腹腔内注射,Danazol对照组每日灌喂Danazol(80mg/kg);生理盐水对照组以与实验组等量的生理盐水腹腔内注射;空白对照组不加干预。观察指标:肉眼观察EM模型的消长及粘连情况,用流式细胞术测定CD3、CD4、CD8T淋巴细胞,用ELISA法测量血清中IL-2、IL-4的含量,大鼠阴道涂片,观察动情周期的变化。【结果】建模后60%的大鼠动情周期发生了改变。用rhPRL治疗后大鼠动情周期全部呈抑制状态,显示出rhPRL对性腺功能抑制。用rhPRL治疗后,异位病灶缩小,粘连受到明显的抑制,与Danazol相比无明显差异(P>0.05),与生理盐水及空白组有显著差异(P<0.05)。用rhPRL治疗后CD4/CD8比值升高,与生理盐水及空白对照组相比有显著差异(P<0.05)。用rhPRL治疗后,大鼠体内白细胞介素IL-4水平下降,而IL-2却明显升高,与生理盐水及空白对照组相比均有显著差异(P<0.05)。【结论】重组人催乳素对大鼠EM模型的生长及粘连有抑制作用,可能是通过对性腺功能的抑制及免疫功能的调节而发挥其作用。[Objective] To investigate the effect of recombinant human prolactin （rhPRL） on endometriosis through giving rhPRL to rat endometriosis model. [Method ] Fifty rat models with endometriosis were established firstly and were seperated into 6 groups： Study group 1 （10 cases） group 2 （10 cases） and group 3 （10 cases）, Danazol control group （10 cases）, 0.9% saline control group （6 cases） and empty control group （4 cases）. Different concentration [50 μg/（kg·d）, 100 μg/（kg·d）, 200 μg/（kg·d）] of rhPRL were injected respectively into the abdominal cavity of study groups. Danazol [80 mg/（kg·d）] were given to Danazol controls. Observation index： diameter of nidus were measured by rider; CD3, CD4, CD8 T lymphocyte were determined by flowcytometry; serum IL-2 and IL-4 were determined by ELISA; the estms period was indirectly observed by pap smear. [Result] Sixty percent of rat estrus were changed after endometriesis were established, which were controlled by rhPRL. Endometriotic niduses shrinked and adhesions were restricted after the injection of rhPRL. There was no statistic difference between study groups and Danazol control group （P 〉0.05）, but there were statistic differences between study groups and 0.9% saline control group and empty control group （P 〈 0.05）. The rate of CD4/CD8 and IL-2 were elevated but IL-4 was lowed in study groups, there were statistic differences between study groups and 0.9% saline control group and empty control group in these indexes （P〈 0.05）.[Conclusion] rhPRL can inhibit the growth and adhesion of endometriosis in rat model through inhibiting the function of gonad and regulating immune function.

关 键 词：子宫内膜异位症 动物模型 重组人催乳素 

分 类 号：R711.7[医药卫生—妇产科学]