自拟中药方治疗脊髓损伤的实验及临床观察  

Experiment and clinical observation of self-drafting traditional Chinese medical prescription for spinal cord injury

作　　者：王建伟[1] 吴毛[1] 马勇[2] 

机构地区：[1]江南大学附属无锡市第五人民医院骨科,江苏省无锡市214073 [2]南京中医药大学骨伤教研室,江苏省南京市210029

出　　处：《中国临床康复》2006年第35期16-19,共4页Chinese Journal of Clinical Rehabilitation

摘　　要：目的:观察自拟中药方-脊髓康治疗脊髓损伤的实验及临床应用效果。方法:动物实验于2003-09/2005-06在南京中医药大学药学院实验室完成,分别进行3个实验:①脊髓康对小鼠棉球肉芽肿质量变化的影响:50只小鼠腋部皮下植入灭菌消毒棉球,缝合后分为5组,即模型组、阿司匹林组、脊髓康(主要由当归、川芎、黄芪、丹参、赤芍等药物组成)12.5g/kg,25g/kg,50g/kg组,每组10只。灌胃给药8d后处死小鼠,取出棉球计算肉芽组织干重。②脊髓康对醋酸所致小鼠腹腔毛细血管通透性增加的影响:50只小鼠,分组同上。静脉注射给药20min后尾静脉注射伊文思蓝生理盐水溶液,然后腹腔注射醋酸,20min后处死各组小鼠,生理盐水反复冲洗腹腔,收集洗涤液离心,测定吸光度值。③脊髓康对Allen法脊髓损伤造模大鼠的抗脊髓损伤作用:60只大鼠分为6组,即假手术组、模型组、地塞米松组、脊髓康12.5g/kg,25g/kg,50g/kg组,每组10只,灌胃给药5d。除假手术组外,麻醉后均行A11en法脊髓损伤大鼠模型制备,假手术组行T9～l0棘突椎板全切除。分别于术后1,3,7,14d按改良的Tarlov标准评价大鼠双后肢神经功能恢复情况(分为5级:1分,没有可觉察的后肢活动;5分,后肢功能完全恢复)。临床观察选择于2003-09/2006-02在江南大学附属无锡市第五人民医院骨科住院的脊髓损伤患者84例,均自愿参加观察。按随机数字表法分为治疗组和对照组,每组42例。对照组常规治疗;治疗组在对照组基础上加用中药脊髓康,50mL口服,2次/d,3周为1个疗程。1个疗程后进行疗效评估:①运动评分:检查身体两侧各自10对肌节中的关键肌,各肌肉的肌力均分为6级,分别记为0～5分:0=完全瘫痪;5=正常肌力。双侧总分为100分。②脊髓损伤程度:采用美国脊髓损伤协会分类标准,共分为5级:A完全性损害,E正常。结果:动物实验结果:①各组小鼠的肉芽组织干重比较:AIM： To observe the experimental and clinical effect with self-made Chinese herb ＂Jisuikang＂ in the treatment of spinal cord injury. METHODS： The animal experiment included 3 parts completed in Laboratory of Pharmacy College, Nanjing University of Traditional Chinese Medicine from September 2003 to June 2005.① The mass effect of ＂Jisuikang＂on mouse tampon granuloma： After implanted the antiseptic tampon in their armpits, 50 mice were divided into 5 groups, namely model group, aspirin group and ＂Jisuikang＂ （composed of angelica root, szechwan lovage rhizome, milkvetch root, danshen root, paeoniae radix and so on） group of three different dose （12.5 g/kg,25 g/kg,50 g/kg） with 10 in each group. After administration for 8 days by gastric perfusion, they were executed to take out the tampoms and calculate the dry weight of granuloma. ②The effect of ＂Jisuikang＂ on the increasing of mouse abdominal cavity capillary permeability caused by acetic acid： 50 mice were divided into 5 groups, too. After 20 minutes of the intravenous injection of medicine, Evans Blue sodium chloride solution was injected through the tail venous, then acetic acid was injected in the abdominal cavity; After the other 20 minutes, the mice were executed and their abdominal cavities were flushed repeatedly with the saline, and then centrifugated cleaning solution which was used to determine absorbance （A）. ③ The reparation effect of ＂Jisuikang＂on the rat＇s spinal cord injury model which was built according to Allen theorem： 60 rats were averagely divided into 6 groups, namely sham operation group, model group, dexamethasone group, ＂Jisuikang＂ group of three different dosage （12.5 g/kg,25 g/kg,50 g/kg） with 10 in each group. The medicine was injected to the rats by gastric perfusion for 5 days. The rat mode of spinal cord injury was built according to Allen theorem after anaesthesia except the sham operation group, which carried on T9-10 laminectomy. The nerve function of the double hind legs

关 键 词：脊髓损伤/中药疗法 神经再生 抗炎药(中药) 镇痛 

分 类 号：R651.21[医药卫生—外科学]