山药苦瓜复方制剂降低2型糖尿病大鼠血糖和血脂的实验(英文)  

Effect of compound preparation of common yam rhizome and balsampear fruit on blood glucose and lipid in rats with type 2 diabetes mellitus

作　　者：张志平[1] 邹丽宜[2] 吴铁[2] 尤婷婷[2] 吴怡[1] 

机构地区：[1]广东医学院科技开发中心,广东省湛江市524023 [2]广东医学院药理教研室,广东省湛江市524023

出　　处：《中国临床康复》2006年第35期180-182,共3页Chinese Journal of Clinical Rehabilitation

基　　金：广东省科技计划资助项目(2003C30501)~~

摘　　要：背景:药食两用中药山药、苦瓜和甘蔗纤维等降糖和降脂作用明显,但作用机制仍不十分清楚。目的:观察山药苦瓜复方制剂对2型糖尿病大鼠血糖、血脂、血胰岛素水平及抗感染能力的影响。设计:随机对照动物实验。单位:广东医学院科技开发中心、药理教研室、实验动物中心、中心实验室。材料:选用SPF级雌性SD大鼠80只,鼠龄4个月。二甲双胍(吉林省东北亚药业股份有限公司,批号040126)。总胆固醇、高密度脂蛋白胆固醇、三酰甘油试剂盒购于北京中生生物技术股份有限公司;SurestepLifescan血糖仪及试纸(美国强生公司);胰岛素放射免疫试剂盒(上海海军医学研究所)鸦UV-3010紫外分光光度计(日本岛津);山药苦瓜复方制剂:由广东医学院科技开发中心提供,批号040321,混悬液,由山药、苦瓜和甘蔗渣膳食纤维等成分经水提而成,其中生药含量1kg/L。方法:实验中的动物饲养和标本采集于2004-06/2005-12在广东医学院实验动物中心和科技开发中心实验室完成,指标的检测在广东医学院药理研究室和中心实验室完成。①随机选取20只为正常对照组,每天灌胃生理盐水5mL/kg。其余60只大鼠灌胃脂肪乳剂5mL/kg,1次/d,连续4周后,大鼠禁食12h,腹腔注射2g/L链脲佐菌素30mg/kg。正常对照组腹腔注射等体积柠檬酸缓冲液。3d后测随机血糖,2周后重复测血糖,以血糖水平高于13.5mmol/L或尿糖>荻以上且持续2周为造模成功(n=48)。②将48只大鼠按随机摸球法分为3组:模型组,二甲双胍组和山药苦瓜复方制剂组,每组16只。模型组灌胃脂肪乳剂5mL/kg;二甲双胍组和山药苦瓜复方制剂组灌胃脂肪乳剂5mL/kg后分别灌胃二甲双胍1mg/kg和含生药1kg/L的山药苦瓜复方制剂5mL/kg。正常对照组:灌胃生理盐水5mL/kg。各组均1次/d,连续6周。③在实验结束前1天测随机血糖。禁食12h用放射免疫法测定血浆胰岛素水平,用分光光�BACKGROUND： Chinese herbs of common yam rhizome, balsampear fruit and bagasse fiber have good effects on decreasing blood glucose and lipid, but its mechanism is unclear. OBJECTIVE： To observe the effect of compound preparation of common yam rhizome and balsampear fruit on blood glucose, lipid, blood insulin and anti-infection of rats with type 2 diabetes mellitus （DM）. DESIGN： Randomized controlled animal study. SETTING： Technological Developing Center, Pharmacological Department, Experimental Animal Center, and Central Laboratory of Guangdong Medical College. MATERIALS： A total of 80 female SD rats with 4 months old and of SPF grade were selected in this study. Flumamine （Jilin Dongbeiya Pharmaceutical Co., Ltd., batch number： 040126）; total cholesterol （TC）, high density lipoprotein cholesterol （HDL-C） and triacylglycerol （TG） （Beijing Zhongsheng Biotechnological Co. Ltd.）; Surestep Life scan blood glucose meter and test paper （Johnson Company, USA）; insulin radio-immunity kit （Shanghai Navy Medical Institute）; UV-3010 ultraviolet spectrophotometer （Japan Shimadzu）; compound preparation of common yam rhizome and balsampear fruit （Technological Developing Center of Guangdong Medical College, batch number： 040321）; suspension was extracted from common yam rhizome, balsampear fruit and dietary fiber of bagasse through water with 1 kg/L raw materials. METHODS： Animal breeding and samples collecting were carried out in the Experimental Animal Center and Technological Developing Central Laboratory of Guangdong Medical College from June 2004 to December 2005; meanwhile, detection of marker was carried out in the Pharmacological Department and Central Laboratory of Guangdong Medical College. ①Twenty rats were randomly selected as normal control group and perfused with 5 mL/kg saline every day. Other 60 rats were perfused with 5 mL/kg fat emulsion once a day for 4 weeks, and then, rats were fasted for 12 hours and peritoneally injected with 2 g/L streptozotocin

关 键 词：糖尿病 非胰岛素依赖型/中药疗法 血糖 脂类/血液 

分 类 号：R587.1[医药卫生—内分泌]