葡萄糖脱氢酶法测定血清葡萄糖的方法学评价  

Evaluation of glucose dehydrogenase method for determination of serum glucose

作　　者：周丽萍[1] 姜旭淦[1] 徐军[2] 

机构地区：[1]江苏大学医学技术学院,江苏镇江212001 [2]江苏大学附属人民医院,江苏镇江212002

出　　处：《江苏大学学报（医学版）》2006年第4期301-303,316,共4页Journal of Jiangsu University：Medicine Edition

基　　金：镇江市科技计划社会发展资助项目(SH2005028)

摘　　要：目的:通过方法学试验对葡萄糖脱氢酶法测定血清葡萄糖方法进行评价。方法:用已建立的方法进行重复性试验、回收试验、干扰试验、线性范围试验,并和HK法进行比较。结果:重复性试验批内CV值为1.33%,批间CV值为1.42%,总CV值为2.01%;回收试验中回收样品I、Ⅱ、Ⅲ的回收率分别为105%,93.5%和91.4%,平均为96.6%;干扰试验表明一般浓度的抗凝剂、防腐剂、尿酸、胆红素对葡萄糖的测定不产生干扰;比较试验,与HK法比较其相关性为Y=1.038X-1.172,r=0.994;线性范围试验表明葡萄糖浓度在20 mmol/L以下呈现良好的线性。结论:葡萄糖脱氢酶法是临床测定血清葡萄糖的较理想的方法。Objective： Glucose dehydrogenase（GDH） method for determination of serum glucose was evaluated through methodology test. Methods： Iteration, recovery,interfere and linely test were tried by an established way,and also compared with HK method. Results： Within-run CV,day-to-day CV and total CV were 1.33% ,1.42% and 2.01% respectively by iterant test; average recovery rate was 96.6% ; anticoagulant,septic,uric acid and bilirubin under a certain concentration also did not interfere with the glucose result in this method. Compared with HK method, the pertinence was Y = 1. 038X - 1. 172, r = 0. 994. The linearity studies showed the method was linear up to 20mmol/L. Conclusion： Glucose dehydrogenase method was a perfect way for determination of serum glucose.

关 键 词：方法学评价 葡萄糖脱氢酶法 葡萄糖 

分 类 号：R446.1[医药卫生—诊断学]