IL-1B及IL-1ra基因多态性与胃癌易患性的相关性研究  

Relationship between Interleukin-1B and Interleukin-1 receptor antagonist gene polymorphisms and susceptibility to gastric cancer

作　　者：高卫[1] 崔霞[1] 胡安拉[1] 邢培祥[2] 孙玉萍[1] 汪运山[1] 

机构地区：[1]山东大学临床医学院济南市中心医院 [2]山东大学齐鲁医院检验科,山东济南250013

出　　处：《江苏大学学报（医学版）》2006年第4期339-341,共3页Journal of Jiangsu University：Medicine Edition

摘　　要：目的:研究白细胞介素-1(IL-1)B和IL-1受体拮抗剂(IL-1 ra)基因多态性与胃癌易患性的关系。方法:应用基因芯片、聚合酶链式反应(PCR)和PCR-限制性片段长度多态性(PCR-RFLP)技术对65例胃癌患者和71例健康对照IL-1B-31和-511及IL-1 ra基因多态性进行了分析。结果:与对照组相比,胃癌患者IL-1B-31T/T携带者、IL-1B-511C/C携带者显著增加(2χ=6.014,P<0.01;2χ=9.533P<0.001)。其他等位基因或基因型未见差异。结论:胃癌的易患性与IL-1B-31TT和IL-1B-511CC基因型可能有关。Objective： To study relationship between interleukin-lB gene and interleukin-1 receptor antagonist gene polymorphisms and susceptibility to gastric cancer. Methods： A total of 71healthy controls and 65 patients with gastric cancer were genotyped by gene chip, polymerase chain reaction （PCR） and PCR- restriction fragment length polymorphism （PCR-RFLP） for IL-1 B-31 and -511 single nucleotide polymorphism （SNP） of IL-1B promoter, and IL-1 ra gene 86bp variable number of tandem repeat（VNTR） poly- morphism of IL-1ra gene intron 2（for five different alleles） Results： Comparison of the IL-1B-31TT genotype and -511 CC genotype frequencies showed a significant difference between patients with gastric cancer and healthy controls（χ^2= 6. 014,P 〈 0.01 ; χ^2 = 9. 533 P 〈 0. 001, respectively）. No association with patients was found for other investigated polymorphisms in the IL-1 B-31 ,-511 and IL-1 ra genes. Conclusion： The results indicated that susceptibility to patients with gastric cancer was increased in individuals carrying both the IL-1B-31TT and IL-1B-511CC genotype.

关 键 词：白细胞介素-1B IL-1受体拮抗剂 基因多态性 基因芯片 胃癌 

分 类 号：R392.3[医药卫生—免疫学] R735.2[医药卫生—基础医学]