检测颈动脉粥样硬化患者血清基质金属蛋白酶的意义  

Meaning of serum matrix metalloproteinase assay in carotid atherosclerosis patients

作　　者：金泽秋 史兵伟 张斌霞 曾晓辉 张奕民 

机构地区：[1]常州市中医院检验科,江苏常州213003 [2]常州市中医院心内科,江苏常州213003 [3]常州市中医院药剂科,江苏常州213003

出　　处：《江苏大学学报（医学版）》2006年第4期342-344,346,共4页Journal of Jiangsu University：Medicine Edition

基　　金：江苏省中医管理局资助项目(9970)

摘　　要：目的:观察颈动脉粥样硬化(CAS)患者血清中基质金属蛋白酶-1(MMP-1)、基质金属蛋白酶-9(MMP-9)及基质金属蛋白酶抑制剂-1(TIMP-1)含量的变化,说明它们与动脉粥样硬化的关系。方法:通过彩色多普勒超声检查及临床判断为CAS患者77例,用高脂饲料加空气干燥术形成家兔颈动脉粥样硬化模型8只。用酶联免疫吸附试验检测CAS患者和家兔模型血清MMP-1、MMP-9及TIMP-1含量,并计数家兔模型血液循环内皮细胞(CEC)水平。结果:CAS患者和家兔模型血清MMP-1、MMP-9水平明显高于正常对照(P<0.05),而TIMP-1水平则低于正常对照(P<0.05)。家兔模型造模4周后开始出现CEC明显增多。血清MMP-1与TIMP-1水平呈负相关。结论:CAS患者血清MMP-1、MMP-9及TIMP-1异常代谢可导致血管内皮破坏,与AS形成密切相关。CAS患者检测这三个指标含量有助于从机理上分析CAS的形成原因,同时可用于观察患者的病变程度和疗效评判。Objective： To observe the level of matrix metalloproteinase-1 （MMP-1）, matrix metalloproteinase-9 （MMP-9） and matrix metallo-proteinase inhibitor-1 （TIMP-1） in carotid atheroselerosis （CAS） patients, to explain the relation between them and carotid atheroselerosis （CAS）. Methods： Seventy-seven CAS patients were diagnosed by color doppler ultrasound, and eight experimental carotid atherosclerotic rabbit models were induced by high cholesterol diet plus air drying. The level of serum MMP-1 , MMP-9, TIMP-1 in CAS patients and experimental carotid atherosclerotic rabbit models were assayed by enzyme linked immunosorbent assay. And the level of blood circulate endothelial cells in rabbit models was assayed. Results： The level of serum MMP-1, MMP-9 in CAS patients and experimental carotid atherosclerotie rabbit models was significantly higher than in the control group （P 〈 0.05）. The level of serum TIMP-1 in CAS patients and experimental crotid atherosclerotic rabbit models was lower than in the control group （P 〈0.05）. The level of blood circulate endothelial cells in rabbit models increased obviously after four weeks. The level of serum MMP-1 was negative correlated with serum TIMP-1. Conclusion： The abnormal metabolism of serum MMP-1, MMP-9 and TIMP-1 could destroy blood vessel endothelium. There was a relation between MMP-1, MMP-9, TIMP-1 and CAS. To assay three important indexes in carotid atherosclerosis cases could help us analyze the reason of CAS, observe the degree of pathological changes and judge curative effect.

关 键 词：颈动脉硬化 基质金属蛋白酶 基质金属蛋白酶抑制剂-1 循环内皮细胞 

分 类 号：R543.5[医药卫生—心血管疾病]