补肾益气中药仙灵脾和黄芪对哮喘大鼠TNF-α和NF-κB的影响  

Effects on Herba Epimedii and Radix Astragali on Tumor Necrosis Factor-α and Nuclear Factor-κB in Asthmatic Rats

作　　者：谢瑾玉[1] 董竞成[1] 宫兆华[1] 赵福东[1] 崔焱[1] 

机构地区：[1]复旦大学附属华山医院中西医结合肺,炎症和肿瘤研究室复旦大学上海医学院中西医结合系,上海200040

出　　处：《中国中西医结合杂志》2006年第8期723-727,共5页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：上海市科委重点资助项目(No.054119648)

摘　　要：目的观察补肾益气中药仙灵脾和黄芪对哮喘大鼠前炎症因子之一的肿瘤坏死因子α(TNFα)和核转录因子κB(NFκB)的影响。方法将大鼠分为5组:生理盐水对照组、哮喘组、中药低剂量组、中药中剂量组、中药高剂量组。ELISA法检测和比较各组大鼠血清中TNFα的浓度,免疫组化法检测和比较各组大鼠肺组织NFκB的活性。结果仙灵脾和黄芪可有效减少哮喘大鼠TNFα的生成,抑制NFκB的活性,中药低、中、高剂量组间比较,差异无显著性。结论在哮喘发作期和缓解期给予仙灵脾和黄芪,可有效减少前炎症因子TNFα的生成,抑制NFκB的活性,抑制炎症的发展。Objective To observe the effects of Herba Epimedii and Radix Astragali, the two Chinese herbs for replenishing Shen and strengthening qi, on tumor necrosis factor-α （TNF-α,one of the pro-inflammatory factors） and nuclear factor-kappa B （NF-κB） in asthmatic rats. Methods Rats were randomly divided into five groups： the normal saline control group, the asthma model group and the three treated groups treated with high, medium and low dose of the Chinese herbs. Serum TNF-α and NF-κB activity in pulmonary tissue were detected with enzyme-linked immunosorbent assay and immunohistochemistry respectively. Results Herba Epimedii and Radix Astragali could effectively reduce the production of TNF-α and inhibit NF-κB activity, and the efficacies in the three treated group were similar, showing insignificant difference among them. Conclusion Application of Herba Epimedii and Radix Astragali in the attack or remission stage of asthma could restrain the development of inflammation by reducing the production of TNF-α and inhibiting NF-κB activity.

关 键 词：补肾益气中药 仙灵脾和黄芪 哮喘 肿瘤坏死因子Α 核转录因子ΚB 

分 类 号：R285[医药卫生—中药学]