肥大软骨细胞的有序凋亡在骨折愈合过程中的潜在作用  

Potential effects of regular apoptosis of hypertrophic chondrocytes in fracture healing

作　　者：陈波[1] 陈林[1] 陈剑[1] 尹良军[1] 苏楠[1] 

机构地区：[1]第三军医大学大坪医院野战外科研究所骨科,重庆400042

出　　处：《创伤外科杂志》2006年第5期441-444,共4页Journal of Traumatic Surgery

基　　金：国家重点基础研究发展规划资助项目(2001CB5103);国家自然科学基金项目(30230370);重庆市科技计划项目(CSTC.2004BA5016)

摘　　要：目的从细胞水平阐明骨折愈合过程中软骨痂向硬骨痂转变演化过程。方法采用小鼠胫骨不稳定骨折模型,对小鼠的骨痂进行X线摄片,HE染色,B rdu掺入检测,ColⅡ、ColX的原位杂交检测,Tunel法对原位凋亡检测。结果骨折修复愈合中后期软骨细胞大量而有序的凋亡,协同邻近编织骨内大量细胞增生侵入才是其主要的演变过程。结论肥大软骨细胞的有序凋亡是干预骨折愈合过程的潜在环节。Objective To clarify the replacement view of cellular level during fracture healing. Methods process of cartilaginous callus by callous callus from the With the instablized fracture models of tibia of mice,the callus were examined by X-ray pictures, hematoxylin-eosin（ HE ） staining, In situ hybridization of Col Ⅱ, Col Ⅹ, Brdu incorporation assay,TdT-mediated dUTP nick end-labeling（ TUNEL） assay. The changes of cartilaginous callus was detected. Results Regular apoptosis of hypertrophic chondrocytes cooperating with mass proliferation of cells in neighboring woven bone is the primary replacement process during fracture healing. Conclusion Regular apoptosis of hypertrophic chondrocytes is the potential step by intervention in fracture healing.

关 键 词：骨折愈合 肥大软骨细胞 凋亡 

分 类 号：R683[医药卫生—骨科学]