Pax2、增殖细胞核抗原、细胞凋亡在肾病综合征激素耐药中的作用  

Effects of Paired Box2,Proliferation Cell Nuclear Antigen and Cell Apoptosis in Nephrotic Syndrome with Steroid-Resistance

作　　者：张慧琼[1] 易著文[2] 何小解[2] 

机构地区：[1]广东医学院附属医院儿科,广东湛江524001 [2]中南大学湘雅二医院儿科,长沙410011

出　　处：《实用儿科临床杂志》2006年第17期1139-1140,1142,共3页Journal of Applied Clinical Pediatrics

摘　　要：目的观察原发性肾病综合征(PNS)激素敏感与激素耐药组患儿Pax2、增殖细胞核抗原(PCNA)表达及细胞有效凋亡率,探讨Pax2在肾脏糖皮质激素(GC)耐药中的作用。方法采用免疫组织化学法观察PNS患儿40例肾活检组织Pax2、PCNA表达,采用荧光显微镜检测细胞凋亡。结果激素敏感组Pax2适量表达,与PCNA阳性表达呈正相关。激素耐药组,Pax2呈过度表达,与PCNA阳性表达无明显相关性。Pax2始终与细胞凋亡呈负相关。结论Pax2适量表达,通过促进细胞增殖及抑制凋亡,修复受损的小管-间质。Pax2过度表达,细胞增殖与凋亡进一步减少,导致肾小管病理改变加重,使其对激素不敏感甚至耐药。Objective To investigate the expression of paired box2（Pax2）,proliferation cell nuclear antigen（PCNA） and cell apoptosis in steroid-sensitive and steroid-resistant groups with primary nephrotic syndrome（PNS） and to find out the action of Pax2 exprcssion in PNS with steroid-resistance. Method The expressions of Pax2, PCNA were evaluated by immunohistochemistry and cell apoptosis by fluorescence micoscope. Results Pax2 expression in renal tubule had a positive correlation with PCNA expression in steroid sensitive group. In steroid-resistant group,Pax2 expression had no correlation with PCNA. Pax2 had a negative correlation with cell apoptosis. Conclusions Pax2 proper expression facilitate PCNA expression and repair tubulointerstitial lesions in steroid - sensitive group. Renal tubular epithelial cell proliferation coordinated with cell apoptosis. Pax2 overexpression in steroid resistant group lead to the decrease of cell proliferation and cell apoptosis and lead to the severe tubule lesions, which made to glucocorticoid resistance.

关 键 词：肾病综合征 PAX2 增殖细胞核抗原 细胞凋亡 药物耐受性 

分 类 号：R726.9[医药卫生—儿科]