结核分支杆菌链霉素耐药基因的杂交检测  

The hybridization detection of the gene of mycobacterium tuberculosis drug resistance of streptomycin

作　　者：潘敏[1] 王晓薇[1] 赵轶[1] 陈海英[2] 韩中波[3] 刘海涛 蒲丹[1] 高宇巍[1] 

机构地区：[1]武警吉林省总队医院,吉林长春130052 [2]吉林大学第一医院,吉林长春130021 [3]吉林市结核病防治研究所,吉林吉林132001 [4]公主岭市疾病控制中心,公主岭136100

出　　处：《中国实验诊断学》2006年第9期1016-1017,共2页Chinese Journal of Laboratory Diagnosis

摘　　要：目的探讨耐链霉素(Sm)结核分支杆菌的编码基因rpsL和rrs的扩增以及突变情况。方法采用PCR扩增技术对23株敏感株,32株耐药株,25例临床结核病人痰标本进行rpsLr、rs基因扩增;并利用寡核苷酸探针反相斑点杂交技术对23株敏感株,32株耐药株,25例临床结核病人痰标本进行rpsLr、rs基因突变检测。结果23株敏感株、32株耐药株rpsLr、rs基因扩增均阳性,阳性率100%;25例临床结核病人痰标本中rpsL基因扩增阳性率为72%,rrs基因扩增阳性率为56%;rpsL基因突变率依次分别为4.3%、65.6%,、50%;rrs基因突变率依次分别为0、12.5%、7.2%。结论耐链霉素(Sm)菌株的基因突变率明显高于药物敏感株;PCR-寡核苷酸探针反相斑点杂交技术以其简便、快速、灵敏的特性能够为临床检测结核分支杆菌对链霉素(Sm)的耐药性提供初步依据。Objective To discuss amplification and mutation about the rpsL and rrs gene of mycobactefium tuberculosis drug resistance of streomycin.Methods Using the polymerase chain reaction（PCR） to indect the rpsL,rrs gene and using the inversedirection nucleotide probe spot hybridize assay to indeet the rpsL, rrs gene mutation of the 23 sensitive cases,32 resistant cases,and 25 sputum samples of tuberculosis patients.Results The positive rate of gene ampllificate of all the 23 sensitive cases,32 resistant cases are 100% ,the positive rate of the rpsL and ms gene in 25 sputum samples is 72% and 56% respectively ;the mutation rate of gene rpsL is 4.3%,65.6%,50% respectively;the mutation rate of gene rrs is0,12.5%,7.2% respectively.Condusion The gene mutation rate of the resistant cases are higher than the sensitive cases. The invemedirection nucleotide probe spot hybridization assay is convenient, quick and sensitive that can provide the basic according in clinical application.

关 键 词：结核分支杆菌 链霉素(Sm) 杂交 

分 类 号：R378.911[医药卫生—病原生物学]