雌二醇对卵巢去势大鼠胃排空的调节作用及其机制研究  

The effect and mechanism study of estradiol on gastric emptying in ovariectomized rats

作　　者：刘然[1] 杨霞[1] 董艳[1] 

机构地区：[1]山东大学齐鲁医院核医学科,山东济南250012

出　　处：《中国实验诊断学》2006年第9期1060-1063,共4页Chinese Journal of Laboratory Diagnosis

摘　　要：目的探讨雌二醇(E2)对卵巢切除大鼠血浆胆囊收缩素(CCK)及胃组织内胆囊收缩素受体A(CCKA)表达水平的影响,以期阐明雌二醇调节胃肠道运动功能的机制。方法给予卵巢切除大鼠E2替代治疗,用放射免疫分析法测定血浆CCK浓度、用99mTc-DTPA液体试餐测定胃排空率,以及用Western blot法检测胃组织内CCKA的表达量。结果E2呈剂量依赖性地抑制卵巢切除大鼠的胃排空,升高血浆CCK的浓度,同时引起胃组织内CCKA受体表达增高。结论雌二醇对卵巢切除大鼠的胃排空具有抑制作用,这种作用是通过促进CCK的分泌以及上调胃内CCKA受体的表达实现的。Objective The effect of estradiol on gastric emptying and the mechanism induced by cholecystokinin and cholecystokinin receptor A were studied in ovariectomized rats. Methods By using estradiol replacement therapy, gastric emptying was assessed in rats 15 min after intragastric instillation of a liquid test meal containing 99 mTc-DTPA; Blood samples were collected for E2 and CCK radioimmunoassay;And expression of CCKA receptor in fundus was assessed by Westem blot. Results E2 could dese-dependently inhibit the gastric emptying and increase the plasma CCK and the expression of gastric CCKA receptor（ P 〈 0.05 respectively）. Conclusion These results indicated that estradiol could inhibit the gastric emptying by increasing the secretion of CCK and the expression of gastric CCKA receptor in ovariectomized rats.

关 键 词：雌二醇 孕酮 胃排空 受体 胆囊收缩素 

分 类 号：R96[医药卫生—药理学]