高效液相色谱法测定复方氯霉素酊中氯霉素含量  

Determination of Chloramphenicol in Chloramphenicol Tincture Compound by HPLC

作　　者：龚银香[1] 魏军 

机构地区：[1]长江大学化学与环境工程学院,湖北荆州434020 [2]湖北省荆州市药品检验所,湖北荆州434000

出　　处：《长江大学学报（自科版）（下旬）》2006年第3期298-299,共2页Journal of Yangtze University

摘　　要：目的：建立测定复方氯霉素酊中氯霉素含量的方法。方法：采用高效液相色谱法，色谱柱为phe—nomenex00g-4097-e0 C18柱（4．6mm×25mm，5μm），流动相为甲醇-水-冰醋酸-三乙胺（100：100：1．5：1．0），流速1mL／min．柱温为40℃．检测波长为278nm。结果：进样量在5=80μg／ml时，与峰面积呈良好线性关系，r=0．9999（n=5）．平均回收率为99．74％，RSD为0．21％（n=5）。结论：该方法准确。重现性好．操作简便，可用于复方氯霉素酊的质量控制。Objective:To establish an analytical method for determination of chloramphenicol in chloramphenicol tincture compound.Method:HPLC was applied,its chromatocolumn was phenomenex 00g-4097-e0 C_ 18 column(4.6 mm×25 mm,5 μm).The mobile phase consisted of CH3OH-H2O-CH3COOH-(CH3CH2)3N(100∶100∶1.5∶1.0),the flow rate was 1ml/min, the column temperature was 40℃ and the UV detection wavelength was 278nm.Results: The linearity was obtained over the range of 5~80 μg/ml(r=0.9999, n=5), the average recovery was 99.74%, RSD was 0.21%(n=5).Conclusion:This method is simple,the result is accurate,the reproducibility is good,and it is reliable and suitable for the determination of chloramphenicol in chloramphenicol tincture compound.

关 键 词：氯霉素酊 氯霉素 高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]