3种茎线虫rDNA区的PCR-RFLP分析  

PCR-RFLP patterns for differentiation of three Ditylenchus species

作　　者：季镭[1] 王金成[2] 杨秀丽[2] 黄国明[2] 林茂松[1] 

机构地区：[1]南京农业大学植物保护学院,江苏南京210095 [2]天津出入境检验检疫局,天津300456

出　　处：《南京农业大学学报》2006年第3期39-43,共5页Journal of Nanjing Agricultural University

基　　金：天津自然科学基金资助项目(05YFJMJC10800)

摘　　要：采用PCR-RFLP技术对来自国内外的10个茎线虫属群体进行了限制性片段长度多态性分析(RFLP),发现甘薯茎线虫(D itylenchus destructor)不同群体间存在基因分化现象。来自山东的2个群体Dell和Dely的酶切图谱与来自其他地区的线虫群体相比明显不同。对甘薯茎线虫的近似种鳞球茎茎线虫(D.dipsaci)以及食菌茎线虫(D.myceliophagus)的分析结果显示,通过ITS-RFLP技术建立的酶切图谱可以很好地将3种茎线虫区分开来。The internal transcribed spacer regions of 10 populations of Ditylenchus were analyzed using PCR-RFLP techniques. The results showed that two ITS-RFLP patterns were found in Ditylenchus destructor and 2 populations of D. destructor from Shandong Province, Dell and Dely, had unique enzyme-digestion pattern compared to other populations of this species. The study also sup- ported that PCR-ITS-RFLP method was a valuable tool for differentiation of D. destructor, D. dipsaci and D. myceliophagus.

关 键 词：甘薯茎线虫 鳞球茎茎线虫 食菌茎线虫 ITS-RFLP 酶切图谱 

分 类 号：S432.4[农业科学—植物病理学]