家蚕病毒性软化病病毒抗血清的研制及应用  

Antiserum Preparation and Application of Infectious Viral Flacherie of Bombyx mori

作　　者：朱宏杰[1] 赵新华 戴建一 吴纯清 王瀛[1] 陆奇能[1] 鲁兴萌[1] 

机构地区：[1]浙江大学动物科学学院蚕蜂研究所,浙江杭州310029 [2]浙江省桐乡市蚕业公司,浙江桐乡314500 [3]浙江省桐乡市蚕业管理站,浙江桐乡314500

出　　处：《蚕桑通报》2006年第3期16-19,共4页Bulletin of Sericulture

基　　金：浙江省科技厅重点科技项目(2003C22013)

摘　　要：从蚕区农户分离了一株家蚕病毒性软化病病毒,暂命名为BmIFV-CHN001。利用蔗糖密度梯度及差速离心法提纯该病毒,将提纯病毒免疫家兔3次,得到BmIFV抗血清。所制备的抗血清采用琼脂双扩散法检测养蚕农户家蚕样本,调查该病毒病在浙江蚕区的感染现状,调查显示农村饲养家蚕具有较高的感染率。Bombyx mori infectious flacherie virus was isolated in the laboratory from Tong-xiang city of Zhejiang province and named BmIFV- CHN001.The virions was purified by differential speed and sucrose. The purified virions immunized rabbit 3 times,getting the antiserum. The presence of BmIFV in Zhejiang was investigated with Gel Immunodiffusion test and the detections showed BmIFV are common in samples of Zhejiang.

关 键 词：家蚕 病毒 软化病 抗血清 

分 类 号：S884.5[农业科学—特种经济动物饲养]