编码微球悬浮芯片与改良ELISA鼠疫杆菌检测方法的比较研究  

Comparative Studies on Coding Microsphere Suspension Chips and Modified ELISA for the Rapid Detection of Yersinia Pestis

作　　者：丁艳丽[1] 王静[1] 侯友松[1] 胡孔新[1] 李伟[1] 陈维娜[1] 

机构地区：[1]中国检验检疫科学研究院卫生检疫研究所,北京100025

出　　处：《中国国境卫生检疫杂志》2006年第B08期35-37,共3页Chinese Journal of Frontier Health and Quarantine

摘　　要：[目的]寻求快速、准确、简便的鼠疫杆菌检测方法。[方法]分别建立鼠疫杆菌编码微球悬浮芯片检测方法和改良ELISA方法，并进行比较。[结果]悬浮芯片对F1抗原最低可以检测到840pg／ml，检测鼠疫杆菌到1×10^3cfu／ml；用改良ELISA法检测，对F1最低可以检测到53ng/ml，检测鼠疫杆菌到1×10^4cfu/ml。[结论]悬浮芯片检测比ELISA具有更高检测灵敏度，并且具有高效、快速、敏感、特异、低成本等优点。Objective To seek a rapid, accurate and convenient method for detecting Yersinia Pestis . Method Two different methods （coding microsphere suspension chips and modified ELISA ） to detect Yersinia Pestis were developed and these two methods were compared. Results Eight hundred and forty pg/ml F1 antigen and 1 ＊10^3cfu/ml Yersinia Pestis were detected by suspension chips and 53 ng/ml F1 antigen and 1×104 cfu/ml Yersinia Pestis were detected by modified ELISA. So the suspension chip had a better sensitivity than the modified ELISA. Conclusion The merits of coding microsphere suspension chip are efficient, rapid, specific, sensitive, lower cost and so on.

关 键 词：编码微球 悬浮芯片 酶联免疫吸附试验 鼠疫杆菌 

分 类 号：R516.8[医药卫生—内科学]