血清乙肝病毒外膜大蛋白检测及其与病毒复制的关系  

Detection of serum hepatitis B virus large envelope protein and its relationship with viral replication

作　　者：戴利成[1] 王翠妮[1] 何建方[1] 钱佳平[2] 王伟洪[2] 施柏年[2] 

机构地区：[1]湖州市中心医院分子医学重点实验室,313000 [2]湖州市中心医院传染科,313000

出　　处：《中华实验和临床病毒学杂志》2006年第3期235-237,共3页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的探讨血清乙型肝炎病毒外膜大蛋白(LHBs)检测对于判定乙型肝炎病毒(HBV)复制的临床意义。方法分别采用ELISA法、时间分辨免疫荧光分析法和实时荧光定量PCR法检测340份慢性乙型肝炎患者血清中LHBs、Pre-S1蛋白、HBV-M和HBVDNA,并进行相关性分析。结果340份慢性乙型肝炎患者血清中LHBs水平与HBVDNA拷贝数变化相一致,两者呈正相关(r=0.899,P=0.038);在不同模式的HBeAg血清中,LHBs与HBVDNA的阳性率差异均无统计学意义(P均>0.05);HBVDNA阳性血清中LHBs阳性率(83.15%)明显高于Pre-S1蛋白和HBeAg的阳性率(50.54%和54.48%),差异有统计学意义(P均<0.05)。结论血清LHBs水平能反映HBV感染者体内HBV复制程度,其灵敏度高于Pre-S1蛋白和HBeAg,可作为判断HBV复制新的血清学指标。Objective To explore the significance of HBV large envelope pretein（LHBs） in diagnosing HBV replication in chronic hepatitis B patients. Methods Serum HBV DNA was quantitively detected by using real-time polymerase chain reaction （RT-PCR）,The LHBs and Pre-S1 were detected by using enzyme linked immunosorbent assay（ELISA） and HBV markers were detected by time differentiate immunofluorescence assay in 340 serum samples collected from chronic hepatitis B patients. Results Serum LHBs level was closely correlated with number of HBV DNA copies （ r = 0.899, P = 0.0380）. There was no significant difference between positive rate of LHBs and that of HBV DNA in different HBeAg pattern （ P 〉 0.05 ） ; the positive rate of LHBs was 83.15 %, which was higher than that of Pre- S1 and HBeAg which were 50.54 % and 54.48 %, respectively. There was significant difference（ P 〈 0.05）. Conclusions The level of serum LHBs can be used to estimate the state of HBV replication and the sensitivity was superior to both Pre-S1 and HBeAg. So it may be used as a new serological marker to detect HBV replication.

关 键 词：肝炎病毒 乙型 病毒包膜蛋白质类 病毒复制 蛋白PreS1 

分 类 号：R373[医药卫生—病原生物学]