罗勒多糖对树突状细胞表面分子表达的影响  

The effect of Qcimum basilicum polysaccharide on the surface molecules expression level of human monocyte-derived dendritic cells

作　　者：郭文菁[1] 杨美香[2] 曲迅[2] 郑广娟[3] 张丹[3] 孔北华[2] 

机构地区：[1]山东大学第二医院妇产科,济南250012 [2]山东大学齐鲁医院临床基础研究所,济南250012 [3]山东中医药大学病理学教研室

出　　处：《中国免疫学杂志》2006年第9期827-829,共3页Chinese Journal of Immunology

基　　金：国家自然科学基金项目资助(30472261)

摘　　要：目的:观察罗勒多糖对人外周血单核细胞来源的树突状细胞(Dendritic cell,DC)表面分子表达的影响,探讨其抗肿瘤免疫机制。方法:从正常人外周血分离获得单核细胞,加入含10%胎牛血清、GM-CSF及IL-4的RPMI1640,37℃培养5天,实验组加入罗勒多糖,对照组加入PBS,流式细胞仪检测细胞表面分子的表达。结果:在细胞因子的诱导下,CD14+单核细胞逐渐分化为DC,罗勒多糖作用组与对照组DC均表达CD209、CD80、CD83、CD86、CD1a和HLA-DR,与对照组相比,罗勒多糖组DC表面分子CD80和HLA-DR的表达均明显上调。结论:罗勒多糖能够调节DC表面分子CD80和HLA-DR的表达,这可能是罗勒多糖发挥其抗肿瘤免疫的机制之一。Objective：To test the affects of a Chinese herb Qcimum basilicum polysaccharide （BP） on the surface molecules expression level of human monocyte-derived dendritic cells. Methods ： Monocytes generated from peripheral blood of healthy donor were cultured for 5 days in 10% FCS-RPMI1640 medium containing GM-CSF and IL-4. BP was added to cells on day 5 of culture for 24 h. The surface molecules expression level of generate monocyte-derived DCs were analysis by FACS. Results ： DC was generated and expressed CD209, CDS0, CD83, CD86, CDla and HLA-DR. BP treated immature DC showed a significant increase in the geometric mean fluorescence intensity of CD80 and HLA-DR. Conclusion：BP is a new antitumor metastasis reagent. Its antitumor metastasis action maybe achieved through regulating some of the antigen presentation related molecules expression level in DC surface.

关 键 词：罗勒多糖 树突状细胞 表面分子 肿瘤 

分 类 号：R392[医药卫生—免疫学]