CD40L、可溶性黏附分子及MMP9在川崎病中的表达及意义  

Significance of T cell CD40L,soluble adhesion molecules and MMP9 in Kawasaki disease

作　　者：王敏[1] 蒋利萍[1] 李秋[1] 李欣[1] 王莉佳[1] 杨锡强[1] 

机构地区：[1]重庆医科大学附属儿童医院免疫室,重庆400014

出　　处：《中国免疫学杂志》2006年第9期867-869,共3页Chinese Journal of Immunology

基　　金：重庆市卫生局科研基金资助(No.99-2062)

摘　　要：目的:研究T细胞CD40配体(CD40L)、血浆中可溶性E选择素(sE-selectin)、可溶性细胞间黏附分子1(sI-CAM-1)和基质金属蛋白酶9(MMP9)在川崎病中的表达及意义。方法:用流式细胞双色荧光标记技术检测20例川崎病急性期及静脉注射免疫球蛋白(IVIG)治疗后的患儿,19例正常对照,外周血T细胞表达CD40L阳性细胞率及平均荧光强度;用ELISA方法检测上述3组血浆中sE-selectin、sICAM-1和MMP9水平。结果:急性期川崎病组T细胞CD40L表达明显高于IVIG治疗后组(P<0.05),川崎病患儿T细胞CD40L表达较正常对照持久;2例伴冠状动脉损害(CAL)的川崎病患儿急性期T细胞CD40L表达高于无CAL者。急性期川崎病组血浆sICAM-1、MMP9明显高于IVIG治疗后组,急性期川崎病患儿血浆sE-se-lectin、sICAM-1明显高于正常对照(P<0.05)。川崎病急性期T细胞表达CD40L与血浆sE-selectin、sICAM-1、MMP9水平无明确相关性(P>0.05)。结论:T细胞表达CD40L升高在川崎病血管炎及冠状动脉病变中可能起一定作用。血浆sE-selectin、sI-CAM-1和MMP9水平可作为反映川崎病血管炎的指标。Objective：To study the significance of T cell CD40L, plasma sE-seleetin, sICAM-1 and MMP9 in the pathogenesis of KD. Methods：The expressions of T cell CD40L were detected by 2-color flow cytometry and the levels of plasma sE-selectin, sICAM-1 and MMP9 were detected by ELISA in 20 KD patients pre and post IVIG, and 19 normal children. Results：The expressions of T cell CD40L were significantly higher compared pre-IVIG with post-IVIG in KD patients （P 〈 0.05 ）. The expressions of T cell CD40L in KD patients were prolonged compared with normal control. The expressions ofT cell CD4.0L in 2 KD patients with coronary artery lesions（CAL） were higher than patients without CAL in the acute phase. The plasma levels of sE-selectin and sICAM-1 in the acute phase of KD patients were elevated in comparison to normal children. Paired analysis of KD patients pre and post IVIG revealed lower levels of sICAM-1 and MMP9（P 〈 0.05 ）. Correlation was not found between T cell CD40L and plasma sE-selectin, sICAM-1 and MMP9（P〉0. 05）. Conclusion：T cell CD40L may probably play a role in the pathogenesis of vasculitis and coronary artery lesions of KD. The plasma levels of sE-selectin, sICAM-1 and MMP9 may be used to reflect the degree of vasculitis of KD.

关 键 词：川崎病 T细胞 CD40配体 可溶性E选择素 可溶性细胞间黏附分子1 基质金属蛋白酶9 

分 类 号：R725.9[医药卫生—儿科]