系统性红斑狼疮患者干扰素调节因子IRF1 IRF4 IRF8基因表达的研究  

The expression of interferon-regulatory factor genes in patients with systemic lupus erythematosus

作　　者：钱捷[1] 沈南[1] 郭桂梅[1] 万年红[2] 林燕[1] 黄新芳[1] 吴慧[3] 陈顺乐[1] 

机构地区：[1]上海交通大学医学院附属仁济医院风湿免疫科上海市风湿病学研究所,200001 [2]上海市闸北区中心医院检验科 [3]中国科学院上海生命科学研究院

出　　处：《中华风湿病学杂志》2006年第9期513-516,共4页Chinese Journal of Rheumatology

基　　金：国家自然科学基金重点资助项目(3000154;30471613;30271224);国家高科技研究发展计划(863计划)基金(2002AA229101);上海市科技发展基金(01JC14029;02QMB1404);上海市科学技术委员会科研计划基金(03DJ14009;05ZR14136);霍英东基金会青年教师基金(81030)

摘　　要：目的研究干扰素调节因子(IRF)家族中IRF1、IRF4、IRF8基因的实时定量表达水平与系统性红斑狼疮(SLE)的关联性。方法收集45例SLE患者与37名正常对照人群的临床资料,取外周血抽提总RNA并反转录为cDNA,以实时荧光定量聚合酶链反应(PCR)方法在ABI PRISM 7900 HT实时定量PCR仪上检测患者组和对照组的IRF1、IRF4、IRF8的mRNA定量表达水平(以-ΔΔCt值表示)的差异。结果正常人外周血IRF1 mRNA、IRF4 mRNA、IRF8 mRNA的表达分别是-3．90±0．19、-8．04±0．25和3．60±0．15。与正常人相比,SLE患者组IRF1 mRNA表达水平差异无统计学意义(P＞0．05),而IRF4 mRNA的表达明显增高(-8．82±0．18,P=0．011)、IRF8 mRNA表达低于正常人(3．09±0．13,P=0．012)。结论SLE患者外周血存在IRF表达异常。Objective To observe whether the expression of interferon-regulatory factor genes are related to systemic lupus erythematosus （SLE）. Methods The clinical data of 45 SLE patients and 37 normal controls were collected. Total RNA of peripheral blood was extracted and transcripted into cDNA. Sybr green dye based real-time quantitative PCR method was used to compare the expression （indicated as -△△Ct value） of IRF1, IRF4, IRF8 in patients with SLE and those in the controls. Results The levels of IRF1, IRF4 and IRF8 mRNA were -3.90±0.19, -8.04±0.25 and 3.60±0.15 respectively in normal controls. In SLE patients, IRF4 mRNA expression was -8.82±0.18, higher than that in normal （P=0.011）. But IRF8 mRNA expression was 3.09±0.13, lower than that in normal （P=-0.012）. Conclusion Abnormal IRF mRNA expression is found in the peripheral blood of SLE patients. IRFs may play roles in the pathogenesis of SLE by affecting the differentiation of Th cells.

关 键 词：红斑狼疮 系统性 聚合酶链反应 干扰素调节因子 

分 类 号：R593.241[医药卫生—内科学]