H1N1亚型猪流感病毒的生物学特性测定及其HA基因序列分析  

Study on Biological Character of Subtype H1N1 Swine influenza virus and Sequence Analysis of HA Gene

作　　者：马祥[1] 顾万军[2] 刘镇明[1] 黄良宗[2] 彭启明[1] 

机构地区：[1]华南农业大学兽医学院,广东广州510642 [2]佛山科技学院,广东佛山528231

出　　处：《动物医学进展》2006年第9期59-62,共4页Progress In Veterinary Medicine

基　　金：广东省自然科学基金项目(34064)

摘　　要：测定了A／Swine／Guangdong／1／01（H1N1）猪流感病毒的部分生物学特性；运用RT—PCR方法扩增其HA基因，并对其进行了克隆和序列分析。结果表明，该株病毒的EID50／0．2mL及TCID50／0．1mL分别为10^-2.3和10^-2.1，小鼠感染试验亦说明该病毒具有致病性。序列分析表明，HA基因全长为1701bp，与国内外流行株同源性达90．8％～99．0％。The biological character of A/Swine/GuangDong/1/01 （H1N1） SIV was studied in this article. The HA gene of SIV was amplified by RT-PCR, then cloned and sequenced to analysis. The result indicated that the EID50/0.2mL and the TCID50/0. 1mL of this sdrain virus were 10^-2.3 and 10^-2.1. The infected test with the rats showed that the virus was pathogentic. At the same time, the sequence analysis indicated that the total length of HA gene was 1702 bp and the homology with the virus in civil and abroad was 90.8% to 99%.

关 键 词：猪流感 逆转录-聚合酶链反应 鸡胚半数感染量 半数的胞感染量 

分 类 号：S852.659.5[农业科学—基础兽医学] Q785[农业科学—兽医学]