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作 者:刘世忠[1] 马玉海[2] 顾为望[1] 潘华[2]
机构地区:[1]南方医科大学实验动物中心,广东广州510515 [2]广州军区疾病预防控制中心,广东广州510507
出 处:《动物医学进展》2006年第9期74-76,共3页Progress In Veterinary Medicine
基 金:总后勤部面上课题(01MB035);广东省科技计划项目(2005B60302007)
摘 要:从比格犬埃立克体阳性血清中提取犬埃立克体IgG,用此IgG免疫鸡制备出抗抗体,用已建立的ELISA检测法对采集的人血清样本进行检测。143份随机采集的人血清4份呈阳性,阳性率2.78%;52份犬场工作人员血清6份呈阳性,阳性率11.54%。结果表明,广州地区犬场工作人员较一般人群犬埃立克体感染率高。To compare Ehrlichia canis infection ratio between ordinary people and people working at canine feed lot in Guangzhou area, we developed a simple, fast method to detect the antibody against Ehrlichia canis in human serum. Immunoglobulin G (IgG) was extracted from serum of Beagle dog experimentally infected with Ehrlichia canis and immunized to laboratory animals for developing antiantibody. 195 seral samples were detected for Ehrlichia canis infection through enzyme-linked immunosorbent assay(ELISA). The result showed that there were obvious difference in seral positive ratio between ordinary people and people working at canine feed lot in Guangzhou area.
分 类 号:S852.64[农业科学—基础兽医学] S858.282[农业科学—兽医学]
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