不同温度和pH对溶组织内阿米巴龟分离株生长的影响  

Effects of Different Temperatures and pH on the Growth of Entamoeba histolytica Strain Isolated from Infected Manouria Impressa

作　　者：郑晶[1] 黄道超[1] 杨光友[1] 姜波[1] 黄红松[1] 牛李丽[2] 王强[2] 邓家波[2] 赵波[2] 陈维刚[2] 余星明[2] 

机构地区：[1]四川农业大学动物科技学院,四川雅安625014 [2]成都动物园,四川成都610041

出　　处：《动物医学进展》2006年第9期77-79,共3页Progress In Veterinary Medicine

基　　金：成都大熊猫繁殖研究基金会资助项目

摘　　要：采用营养琼脂双相培养基对溶组织内阿米巴龟分离株进行了不同温度（25，30，34，37℃和40℃）和PH（1．0，2．0，3．0，4．0，5．0，6．0，7．0，8．0，9．0和10．0）环境条件下的体外培养观察。结果表明，溶组织内阿米巴龟分离株在25℃～40℃之间均可生长，最适生长温度为37℃；在PH1～2之间不生长，pH3～10之间可以生长，最适生长范围为pH5-8。Entamoeba histolytica strain was isolated from infected Manouria impressa and established on LAS mediumin vitro. The growth of islated strain under different culture condition, including temperatures （25,30,34,37 ℃ and 40 ℃） and pH （1.0,2.0,3.0,4.0,5.0,6.0,7.0,8.0,9.0,10.0） was observed. The results showed that isolated strain could grow in the temperature range between 25 ℃ to 40 ℃. The optimum temperature was 37 ℃. It also could grow on the pH range of 3.0 to 10.0,but it could not grow on the range of 1.0 to 3.0 . The optimum pH range was 5.0 to 8.0 .

关 键 词：溶组织内阿米巴 凹甲陆龟 体外培养 温度 PH 

分 类 号：S852.721[农业科学—基础兽医学] S854.43[农业科学—兽医学]